제품 > 항체 > 다중클론항체(pAb)

[KO Validated] IRF9 Rabbit pAb (A21331)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - [KO Validated] IRF9 Rabbit pAb (A21331)

Western blot analysis of extracts of various cell lines, using IRF9 antibody (A21331) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KO Validated] IRF9 Rabbit pAb (A21331)

Western blot analysis of extracts from normal (control) and IRF9 knockout (KO) A549 cells, using IRF9 Rabbit pAb (A21331) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name[KO Validated] IRF9 Rabbit pAb
Catalog No.A21331
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Mutations in this gene result in Immunodeficiency 65.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 113-255 of human IRF9 (NP_006075.3).
SequenceQLLPPGIVSGQPGTQKVPSKRQHSSVSSERKEEEDAMQNCTLSPSVLQDSLNNEEEGASGGAVHSDIGSSSSSSSPEPQEVTDTTEAPFQGDQRSLEFLLPPEPDYSLLLTFIYNGRVVGEAQVQSLDCRLVAEPSGSESSME
Gene ID
Swiss Prot
Synonymsp48; IRF-9; ISGF3; ISGF3G
Calculated MW44kDa
Observed MW43kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA549
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] IRF9 Rabbit pAb (A21331)}

Western blot - [KO Validated] IRF9 Rabbit pAb (A21331)

Western blot analysis of extracts of various cell lines, using IRF9 antibody (A21331) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KO Validated] IRF9 Rabbit pAb (A21331)}

Western blot - [KO Validated] IRF9 Rabbit pAb (A21331)

Western blot analysis of extracts from normal (control) and IRF9 knockout (KO) A549 cells, using IRF9 Rabbit pAb (A21331) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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