제품 > 항체 > 단일클론항체(mAb)

[KO Validated] Lamin A/C Rabbit mAb (A19524)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)

The STORM super-resolution (SR) imaging of U-2 OS cells using [KO Validated] Lamin A/C Rabbit mAb (A19524, ABclonal) at dilution of 1:200 with 3% paraformaldehyde (PFA) +0.1% glutaraldehyde (GA) fixation. The immunostaining was performed by Full Automatic Immunofluorescence Workflow System (Workflow Ultra300, Nano-Micro imaging, China). Image was performed with Single-Molecule Localization Super-Resolution Microscopy (STORM Ultra300, Nano-Micro imaging, China). We acknowledge Nano-Micro imaging Biotechnology Co., Ltd. in SR image processing and kindly providing this image.

Immunofluorescence analysis of H9C2 cells using [KO Validated] Lamin A/C Rabbit mAb (A19524) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using [KO Validated] Lamin A/C Rabbit mAb (A19524) at dilution of 1:200. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of HeLa cells using [KO Validated] Lamin A/C Rabbit mAb (A19524) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using [KO Validated] Lamin A/C Rabbit mAb (A19524) at dilution of 1:100 - 1:1600. Blue: DAPI for nuclear staining.

ABclonal:Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] Lamin A/C Rabbit mAb (A19524) at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using [KO Validated] Lamin A/C Rabbit mAb (A19524) at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] Lamin A/C Rabbit mAb (A19524) at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KO Validated] Lamin A/C Rabbit mAb (A19524) at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KO Validated] Lamin A/C Rabbit mAb (A19524) at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using [KO Validated] Lamin A/C Rabbit mAb (A19524) at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Western blot analysis of lysates from wild type (WT) and Lamin A/C knockout (KO) HeLa cells using [KO Validated] Lamin A/C Rabbit mAb (A19524) at 1:50000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from A549 cells using [KO Validated] Lamin A/C Rabbit mAb (A19524) at 1:240000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of various lysates using [KO Validated] Lamin A/C Rabbit mAb (A19524) at 1:300000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Confocal imaging of U-2 OS cells using [KO Validated] Lamin A/C Rabbit mAb (A19524, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunoprecipitation - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunoprecipitation analysis of 300 μg extracts from PC-12 cells using 3 μg [KO Validated] Lamin A/C Rabbit mAb (A19524). Western blot was performed from the immunoprecipitate using [KO Validated] Lamin A/C Rabbit mAb (A19524) at a dilution of 1:100000.

Review

Overview

Product name	[KO Validated] Lamin A/C Rabbit mAb
Catalog No.	A19524
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC5001-08

Background

The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A/C (NP_733821.1).
Sequence	SSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSS
Gene ID
Swiss Prot
Synonyms	FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B; /C
Calculated MW	74kDa
Observed MW	68kDa/72kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:50000 - 1:300000 IHC-P 1:1000 - 1:4000 IF/ICC 1:100 - 1:800 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	HeLa, A-549, NIH/3T3, PC-12
Cellular location	Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm.
Customer validation	(HEK293T cells, Caenorhabditis Elegans) WB(Mus musculus, C. elegans, Homo sapiens, Rattus norvegicus) IF(Mus musculus, Homo sapiens) IF(Homo sapiens) IP(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] Lamin A/C Rabbit mAb images

Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunofluorescence analysis of H9C2 cells using [KO Validated] Lamin A/C Rabbit mAb (A19524) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunofluorescence analysis of L929 cells using [KO Validated] Lamin A/C Rabbit mAb (A19524) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Confocal immunofluorescence analysis of HeLa cells using [KO Validated] Lamin A/C Rabbit mAb (A19524) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunofluorescence analysis of U-2 OS cells using [KO Validated] Lamin A/C Rabbit mAb (A19524) at dilution of 1:100 - 1:1600. Blue: DAPI for nuclear staining.

Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunoprecipitation - [KO Validated] Lamin A/C Rabbit mAb (A19524)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A19524

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Product Protocols

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Theranostics

Application:

IF:

11.55

Species:

Mus musculus

PMID:

33897879

Title:

Radiation-induced abscopal reproductive effect is driven by TNF-α/p38 MAPK/Rac1 axis in Sertoli cells

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Exp Cell Res

Application:

IF:

3.9

Species:

Mus musculus

PMID:

34052237

Title:

Vimentin loss promotes cancer proliferation through up-regulating Rictor/AKT/β-catenin signaling pathway

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Neoplasia

Application:

IF:

5.715

Species:

Mus musculus

PMID:

34153644

Title:

Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Cancer letters

Application:

IF:

9.75

Species:

Mus musculus

PMID:

37217071

Title:

AFP deletion leads to anti-tumorigenic but pro-metastatic roles in liver cancers with concomitant CTNNB1 mutations

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

The Journal of cell biology

Application:

IF:

8.07

Species:

C. elegans

PMID:

37058288

Title:

Lysosomal chloride transporter CLH-6 protects lysosome membrane integrity via cathepsin activation

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Mus musculus

PMID:

37689690

Title:

Chemical-induced phase transition and global conformational reorganization of chromatin - PMC

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Cell reports

Application:

IF:

Species:

Mus musculus

PMID:

36198274

Title:

Destabilization of TP53 by USP10 is essential for neonatal autophagy and survival

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Nucleic acids research

Application:

WB, IF

IF:

19.16

Species:

Homo sapiens

PMID:

35929067

Title:

Transcription-coupled donor DNA expression increases homologous recombination for efficient genome editing

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Cell reports

Application:

IF:

Species:

Homo sapiens,Mus musculus

PMID:

38308845

Title:

ABHD7-mediated depalmitoylation of lamin A promotes myoblast differentiation

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Acta Pharm Sin B

Application:

IF:

14.903

Species:

Mus musculus

PMID:

34729308

Title:

Cholesterol-associated lysosomal disorder triggers cell death of hematological malignancy: Dynamic analysis on cytotoxic effects of LW-218

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Journal of virology

Application:

IF:

6.54

Species:

Mus musculus

PMID:

35442061

Title:

Porcine Epidemic Diarrhea Virus nsp7 Inhibits Interferon-Induced JAK-STAT Signaling through Sequestering the Interaction between KPNA1 and STAT1

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Free radical biology & medicine

Application:

IF:

7.4

Species:

Rattus norvegicus

PMID:

38582228

Title:

Doxorubicin induces phosphorylation of lamin A/C and loss of nuclear membrane integrity: A novel mechanism of cardiotoxicity

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Cell death and differentiation

Application:

IF:

12.4

Species:

Homo sapiens

PMID:

38664591

Title:

The cGAS-Ku80 complex regulates the balance between two end joining subpathways

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Aging cell

Application:

IF:

7.8

Species:

Homo sapiens

PMID:

38482753

Title:

Mitophagy defect mediates the agingassociated hallmarks in HutchinsonGilford progeria syndrome

References for [KO Validated] Lamin A/C Rabbit mAb (A19524)

Product:

[KO Validated] Lamin A/C Rabbit mAb

Journal:

Frontiers in bioengineering and biotechnology

Application:

IF:

4.3

Species:

Homo sapiens

PMID:

39493304

Title:

Extracellular vesicle mimetics as delivery vehicles for oligonucleotide-based therapeutics and plasmid DNA - PMC

Publishing research using A19524? Please let us know so that we can cite the reference in this datasheet.

항체 (5)

Secondary Antibodies (26)

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제품 > 항체 > 단일클론항체(mAb)

[KO Validated] Lamin A/C Rabbit mAb (A19524)

Overview

Background

Immunogen information

Applications

Documents

Certificate of Compliance

Research Area

[KO Validated] Lamin A/C Rabbit mAb images

Submit an review for A19524

Reviews List

Product Protocols

항체 (5)

Secondary Antibodies (26)