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[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from 293T cells, using [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from wild type (WT) and NADPH oxidase 4 (NOX4) knockout (KO) HeLa cells, using [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse colon using [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat colon using [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb
Catalog No.	A11274
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 328-578 of human NADPH oxidase 4 (NOX4) (NP_058627.1).
Sequence	KENFKARPGQYITLHCPSVSALENHPFTLTMCPTETKATFGVHLKIVGDWTERFRDLLLPPSSQDSEILPFIQSRNYPKLYIDGPFGSPFEESLNYEVSLCVAGGIGVTPFASILNTLLDDWKPYKLRRLYFIWVCRDIQSFRWFADLLCMLHNKFWQENRPDYVNIQLYLSQTDGIQKIIGEKYHALNSRLFIGRPRWKLLFDEIAKYNRGKTVGVFCCGPNSLSKTLHKLSNQNNSYGTRFEYNKESFS
Gene ID
Swiss Prot
Synonyms	KOX; KOX-1; RENOX; 4)
Calculated MW	67kDa
Observed MW	65kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	293T
Cellular location	Cell junction, Cell membrane, Endoplasmic reticulum membrane, Multi-pass membrane protein, Nucleus, focal adhesion, nucleolus.
Customer validation	WB(Mus musculus, Homo sapiens, Rattus norvegicus, Danio rerio, Rattus norvegicus) IHC(Mus musculus, Rattus norvegicus) IF(Mus musculus, Rattus norvegicus, Homo sapiens) IHC(Homo sapiens, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb images

Western blot - [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Immunohistochemistry - [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Chemico-biological interactions

Application:

IF:

5.192

Species:

Mus musculus

PMID:

31153982

Title:

Hesperetin relieves cisplatin-induced acute kidney injury by mitigating oxidative stress, inflammation and apoptosis.

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Biochemical Pharmacology

Application:

IF:

5.858

Species:

Homo sapiens,Rattus norvegicus

PMID:

32251672

Title:

Dipeptidyl peptidase-4 inhibition improves endothelial senescence by activating AMPK/SIRT1/Nrf2 signaling pathway

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Frontiers in Cellular Neuroscience

Application:

IF:

5.505

Species:

Rattus norvegicus

PMID:

30983975

Title:

The Neuroprotective Effect of Astaxanthin on Pilocarpine-Induced Status Epilepticus in Rats.

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Adipocyte

Application:

IF:

4.534

Species:

Homo sapiens

PMID:

31718404

Title:

Increased oxidative stress, inflammation and fibrosis in perirenal adipose tissue of patients with cortisol-producing adenoma.

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Frontiers in Cellular and Infection Microbiology

Application:

IF:

5.293

Species:

Homo sapiens

PMID:

31921708

Title:

Hepatitis E Virus Induces Brain Injury Probably Associated With Mitochondrial Apoptosis.

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

J Cell Mol Med

Application:

IF:

5.31

Species:

Homo sapiens

PMID:

32705806

Title:

Andrographolide attenuates epithelial‐mesenchymal transition induced by TGF‐β1 in alveolar epithelial cells

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Food Funct

Application:

IF:

5.396

Species:

Mus musculus

PMID:

32377661

Title:

Pterostilbene prevents LPS-induced early pulmonary fibrosis by suppressing oxidative stress, inflammation and apoptosis in vivo

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Acta Pharmacol Sin

Application:

IF:

6.15

Species:

Mus musculus

PMID:

33654217

Title:

Roxithromycin attenuates bleomycin-induced pulmonary fibrosis by targeting senescent cells

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

J Biol Chem

Application:

IF:

5.157

Species:

Mus musculus

PMID:

33158991

Title:

Adipose-specific BMP and Activin Membrane-Bound Inhibitor (BAMBI) Deletion Promotes Adipogenesis by Accelerating ROS Production

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

J Hazard Mater

Application:

IF:

10.58

Species:

Homo sapiens

PMID:

34492938

Title:

Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Life Sci

Application:

IF:

5.037

Species:

Rattus norvegicus

PMID:

34090859

Title:

Daidzein mitigates myocardial injury in streptozotocin-induced diabetes in rats

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Int J Biol Sci

Application:

IF:

6.58

Species:

Mus musculus

PMID:

34803511

Title:

Co-targeting BET bromodomain BRD4 and RAC1 suppresses growth, stemness and tumorigenesis by disrupting the c-MYC-G9a-FTH1axis and downregulating HDAC1 in molecular subtypes of breast cancer

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Endocrine-related cancer

Application:

IF:

5.9

Species:

Homo sapiens

PMID:

32856615

Title:

Role of PDK4 in insulin signaling pathway in periadrenal adipose tissue of pheochromocytoma patients

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

European journal of pharmacology

Application:

IF:

5.19

Species:

Mus musculus

PMID:

35398392

Title:

Oridonin attenuates LPS-induced early pulmonary fibrosis by regulating impaired autophagy, oxidative stress, inflammation and EMT

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Free radical biology & medicine

Application:

IF:

8.1

Species:

Rattus norvegicus

PMID:

35513128

Title:

Up-regulation of Nrf2/HO-1 and inhibition of TGF-β1/Smad2/3 signaling axis by daphnetin alleviates transverse aortic constriction-induced cardiac remodeling in mice

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Molecular and cellular endocrinology

Application:

IF:

4.1

Species:

Danio rerio

PMID:

38342135

Title:

Participation of follicular superoxides, inflammatory modulators, and endocrine factors in zebrafish (Danio rerio) ovulation: Cross-talk between PKA and MAPK

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Homo sapiens

PMID:

38493213

Title:

Therapeutic targeting nudix hydrolase 1 creates a MYC-driven metabolic vulnerability

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Free Radic Biol Med

Application:

WB, IHC, IF

IF:

8.101

Species:

Mus musculus

PMID:

34298093

Title:

Iron deficiency exacerbates cisplatin-or rhabdomyolysis-induced acute kidney injury through promoting iron-catalyzed oxidative damage

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Front Pharmacol

Application:

WB, IHC, IF

IF:

5.988

Species:

Mus musculus

PMID:

34588976

Title:

N-Acetyl Cysteine Ameliorates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease and Intracellular Triglyceride Accumulation by Preserving Mitochondrial Function

References for [KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb (A11274)

Product:

[KO Validated] NADPH oxidase 4 (NOX4) Rabbit pAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

WB, IHC, IF

IF:

7.67

Species:

Mus musculus, Rattus norvegicus

PMID: