제품 > 항체 > 단일클론항체(mAb)

[KO Validated] N-Cadherin Rabbit mAb (A19083)

KO/KDValidated

Publications (96) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of lysates from wild type (WT) and N-Cadherin knockout (KO) HeLa cells using [KO Validated] N-Cadherin Rabbit mAb (A19083) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of lysates from C2C12 cells using [KO Validated] N-Cadherin Rabbit mAb (A19083) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of lysates from C6 cells using [KO Validated] N-Cadherin Rabbit mAb (A19083) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] N-Cadherin Rabbit mAb (A19083) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using [KO Validated] N-Cadherin Rabbit mAb (A19083) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using [KO Validated] N-Cadherin Rabbit mAb (A19083) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KO Validated] N-Cadherin Rabbit mAb (A19083) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Confocal imaging of paraffin-embedded rat heart using [KO Validated] N-Cadherin Rabbit mAb (A19083, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Confocal imaging of paraffin-embedded Mouse heart using [KO Validated] N-Cadherin Rabbit mAb (A19083, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

Overview

Product name[KO Validated] N-Cadherin Rabbit mAb
Catalog No.A19083
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0371
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human N Cadherin (P19022).
SequenceAKFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVIPPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGP
Gene ID
Swiss Prot
SynonymsCDHN; NCAD; ACOGS; ADHD8; CD325; ARVD14; CDw325; in
Calculated MW100kDa
Observed MW140kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, C2C12, C6
Cellular locationCell membrane, Single-pass type I membrane protein.
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa, Rattus norvegicus, Bos taurus)

IF(Homo sapiens)

IHC(Homo sapiens, Mus musculus)

IF(Homo sapiens)

IHC(Homo sapiens, Mus musculus)

IP(Homo sapiens)

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)}

Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of lysates from wild type (WT) and N-Cadherin knockout (KO) HeLa cells using [KO Validated] N-Cadherin Rabbit mAb (A19083) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)}

Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of lysates from C2C12 cells using [KO Validated] N-Cadherin Rabbit mAb (A19083) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)}

Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of lysates from C6 cells using [KO Validated] N-Cadherin Rabbit mAb (A19083) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)}

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] N-Cadherin Rabbit mAb (A19083) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)}

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using [KO Validated] N-Cadherin Rabbit mAb (A19083) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)}

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using [KO Validated] N-Cadherin Rabbit mAb (A19083) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)}

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KO Validated] N-Cadherin Rabbit mAb (A19083) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083)}

Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Confocal imaging of paraffin-embedded rat heart using [KO Validated] N-Cadherin Rabbit mAb (A19083, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083)}

Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Confocal imaging of paraffin-embedded Mouse heart using [KO Validated] N-Cadherin Rabbit mAb (A19083, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A19083? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)