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[KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Monkey

Western blot analysis of various lysates using [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from wild type(WT) and NF-kB p65/RelA knockout (KO) HeLa cells, using [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Confocal imaging of HeLa cells using [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331, dilution 1:100) (Red). The cells were counterstained with MonoMethyl-Histone H3-K4 Rabbit mAb (A22078, dilution 1:300) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

ABclonal:Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Immunoprecipitation of [KO Validated] NF-kB p65/RelA Rabbit mAb from 500 µg extracts of HeLa cells was performed using 2 µg of [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331) at a dilution of 1:10000.

ABclonal:Chromatin Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Chromatin immunoprecipitation analysis of extracts of HT-1080 cells, HT-1080 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes, using NF-kB p65/RelA antibody (A22331) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product name	[KO Validated] NF-kB p65/RelA Rabbit mAb
Catalog No.	A22331
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC51088

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 450-551 of human NF-kB p65/RelA (NP_068810.3).
Sequence	LGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS
Gene ID
Swiss Prot
Synonyms	p65; CMCU; NFKB3; AIF3BL3; lA
Calculated MW	58kDa/59kDa/60kDa
Observed MW	65kDa/65KD

Applications

Reactivity	Human, Mouse, Rat, Monkey
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:10000 - 1:60000 IHC-P 1:200 - 1:2000 IF/ICC 1:100 - 1:800 IP 0.5μg-4μg antibody for 200μg-500μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP 5μg antibody for 10μg-15μg of Chromatin
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	HeLa, PC-12, NIH/3T3, Mouse lung, HeLa
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Homo sapiens, Rattus norvegicus, Mus musculus) Co-IP(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] NF-kB p65/RelA Rabbit mAb images

Western blot - [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Immunohistochemistry - [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Immunofluorescence - [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Chromatin Immunoprecipitation - [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Biotechnology & genetic engineering reviews

Application:

IF:

4.2

Species:

Homo sapiens

PMID:

36718919

Title:

Antiviral and anti-inflammatory activity of novel belladonna formulation against Japanese encephalitis virus via inhibition of p65 nuclear translocation and TNF-α …

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Toxicology and applied pharmacology

Application:

IF:

4.46

Species:

Rattus norvegicus

PMID:

37328117

Title:

4-Terpineol attenuates pulmonary vascular remodeling via suppressing PI3K/Akt signaling pathway in hypoxia-induced pulmonary hypertension rats

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Homo sapiens

PMID:

37864909

Title:

miRNA transcriptomics analysis shows miR-483-5p and miR-503-5p targeted miRNA in extracellular vesicles from severe acute pancreatitis-associated lung injury

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Bioorganic chemistry

Application:

IF:

5.3

Species:

Mus musculus

PMID:

38367429

Title:

Diterpenoids from Euphorbia peplus possessing cytotoxic and anti-inflammatory activities

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Physiological research

Application:

IF:

2.1

Species:

Mus musculus

PMID:

38466005

Title:

Eicosapentaenoic Acid Alleviates Inflammatory Response and Insulin Resistance in Pregnant Mice With Gestational Diabetes Mellitus

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Acta biomaterialia

Application:

IF:

9.7

Species:

Mus musculus

PMID:

38458511

Title:

Tumor-targeted Delivery of Copper-manganese Biomineralized Oncolytic Adenovirus for Colorectal Cancer Immunotherapy

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

International journal of biological sciences

Application:

Co-IP

IF:

10.75

Species:

Mus musculus

PMID:

37928255

Title:

Genipin-activating PPAR impedes CCR2-mediated macrophage infiltration into postoperative liver to suppress recurrence of hepatocellular carcinoma - PMC

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Brain research bulletin

Application:

IF:

3.8

Species:

Mus musculus

PMID:

38810789

Title:

JR14a: A Novel Antagonist of C3aR Attenuates Neuroinflammation in Cerebral Ischemia-Reperfusion Injury

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Molecular nutrition & food research

Application:

IF:

5.2

Species:

Mus musculus

PMID:

38778506

Title:

Dietary Oat Glucan Alleviates HighFat Induced Insulin Resistance through Regulating Circadian Clock and Gut Microbiome

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

Autophagy

Application:

IF:

13.3

Species:

Mus musculus

PMID:

38910554

Title:

(-)-epigallocatechin 3-gallate protects pancreatic -cell against excessive autophagy-induced injury through promoting FTO degradation

References for [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331)

Product:

[KO Validated] NF-kB p65/RelA Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

38442575

Title:

Identification of Treg-related prognostic molecular subtypes and individualized characteristics in clear cell renal cell carcinoma through single-cell transcriptomes and