제품 > 항체 > 다중클론항체(pAb)

[KO Validated] SIRT3 Rabbit pAb (A5718)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse liver, using [KO Validated] SIRT3 Rabbit pAb (A5718) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of various lysates using [KO Validated] SIRT3 Rabbit pAb (A5718) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from wild type(WT) and SIRT3 knockout (KO) 293T cells, using [KO Validated] SIRT3 Rabbit pAb (A5718) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] SIRT3 Rabbit pAb (A5718)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using [KO Validated] SIRT3 Rabbit pAb (A5718) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using [KO Validated] SIRT3 Rabbit pAb (A5718) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] SIRT3 Rabbit pAb (A5718) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] SIRT3 Rabbit pAb (A5718)

Immunofluorescence analysis of NIH/3T3 cells using SIRT3 Rabbit pAb (A5718) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	[KO Validated] SIRT3 Rabbit pAb
Catalog No.	A5718
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. SIRT3 has far-reaching effects on nuclear gene expression, cancer, cardiovascular disease, neuroprotection, aging, and metabolic control.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 299-399 of human SIRT3 (NP_036371.1).
Sequence	PQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK
Gene ID
Swiss Prot
Synonyms	SIR2L3; T3
Calculated MW	44kDa
Observed MW	28kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:20 - 1:50 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HepG2, HeLa, 293T, Mouse liver
Cellular location	Mitochondrion matrix.
Customer validation	WB(Homo sapiens, Mus musculus, Rattus norvegicus) Co-IP(Mus musculus) IF(Rattus norvegicus) IF(Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] SIRT3 Rabbit pAb images

Western blot - [KO Validated] SIRT3 Rabbit pAb (A5718)

Immunohistochemistry - [KO Validated] SIRT3 Rabbit pAb (A5718)

Immunofluorescence - [KO Validated] SIRT3 Rabbit pAb (A5718)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] SIRT3 Rabbit pAb (A5718)

Product:

[KO Validated] SIRT3 Rabbit pAb

Journal:

Cell Cycle

Application:

IF:

3.4

Species:

Homo sapiens

PMID:

25607651

Title:

SIRT6 rescues the age related decline in base excision repair in a PARP1-dependent manner

References for [KO Validated] SIRT3 Rabbit pAb (A5718)

Product:

[KO Validated] SIRT3 Rabbit pAb

Journal:

Clinical science (London, England : 1979)

Application:

IF:

6.7

Species:

Mus musculus

PMID:

31383716

Title:

Mitochondrial ROS-induced lysosomal dysfunction impairs autophagic flux and contributes to M1 macrophage polarization in a diabetic condition.

References for [KO Validated] SIRT3 Rabbit pAb (A5718)

Product:

[KO Validated] SIRT3 Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.6

Species:

Mus musculus

PMID:

32848753

Title:

Trimetazidine Inhibits Renal Tubular Epithelial Cells to Mesenchymal Transition in Diabetic Rats via Upregulation of Sirt1

References for [KO Validated] SIRT3 Rabbit pAb (A5718)

Product:

[KO Validated] SIRT3 Rabbit pAb

Journal:

J Food Biochem

Application:

IF:

Species:

Rattus norvegicus

PMID:

34132394

Title:

Piceatannol alleviate ROS‐mediated PC‐12 cells damage and mitochondrial dysfunction through SIRT3/FOXO3a signaling pathway

References for [KO Validated] SIRT3 Rabbit pAb (A5718)

Product:

[KO Validated] SIRT3 Rabbit pAb

Journal:

Molecular metabolism

Application:

IF:

Species:

Mus musculus

PMID:

35944895

Title:

The mitochondrial NAD kinase functions as a major metabolic regulator upon increased energy demand - PMC

References for [KO Validated] SIRT3 Rabbit pAb (A5718)

Product:

[KO Validated] SIRT3 Rabbit pAb

Journal:

The Journal of dermatology

Application:

IF:

3.1

Species:

Homo sapiens

PMID:

36412048

Title:

GATA3 ameliorates melanocyte injuries in vitiligo through SIRT3‐mediated HMGB1 deacetylation

References for [KO Validated] SIRT3 Rabbit pAb (A5718)

Product:

[KO Validated] SIRT3 Rabbit pAb

Journal:

Stem cells international

Application:

Co-IP

IF:

3.8

Species:

Mus musculus

PMID:

35386849

Title:

Mitofusin2 Promotes Cell Maturation from Mouse Embryonic Stem Cells via Sirt3/Idh2 Activation

References for [KO Validated] SIRT3 Rabbit pAb (A5718)

Product:

[KO Validated] SIRT3 Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

WB, IF

IF:

5.6

Species:

Rattus norvegicus

PMID:

35662706

Title:

Superoxide dismutase@zeolite Imidazolate Framework-8 Attenuates Noise-Induced Hearing Loss in Rats - PMC

References for [KO Validated] SIRT3 Rabbit pAb (A5718)

Product:

[KO Validated] SIRT3 Rabbit pAb

Journal:

Free radical biology & medicine

Application:

IF:

7.1

Species:

Mus musculus

PMID:

38008130

Title:

Enhancement of mitochondrial energy metabolism by melatonin promotes vascularized skeletal muscle regeneration in a volumetric muscle loss model

References for [KO Validated] SIRT3 Rabbit pAb (A5718)

Product:

[KO Validated] SIRT3 Rabbit pAb

Journal:

CNS neuroscience & therapeutics

Application:

IF:

5.5

Species:

Mus musculus

PMID:

39123294

Title:

Hyperglycemia-induced Sirt3 downregulation increases microglial aerobic glycolysis and inflammation in diabetic neuropathic pain pathogenesis - PMC

References for [KO Validated] SIRT3 Rabbit pAb (A5718)

Product:

[KO Validated] SIRT3 Rabbit pAb

Journal:

Biochemical pharmacology

Application:

IF:

5.3

Species:

Rattus norvegicus

PMID:

39134283

Title:

Trimetazidine Attenuates Ischemia/Reperfusion-Induced Myocardial Ferroptosis by Modulating the Sirt3/Nrf2-GSH System and Reducing Mitochondrial Oxidative