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[KO Validated] SND1 Rabbit pAb (A5874)

KO/KDValidated

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] SND1 Rabbit pAb (A5874)

Western blot analysis of various lysates using [KO Validated] SND1 Rabbit pAb (A5874) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] SND1 Rabbit pAb (A5874)

Western blot analysis of various lysates using [KO Validated] SND1 Rabbit pAb (A5874) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] SND1 Rabbit pAb (A5874)

Western blot analysis of various lysates, using SND1 Rabbit pAb (A5874) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] SND1 Rabbit pAb (A5874)

Western blot analysis of lysates from wild type (WT) and SND1 knockout (KO) HeLa cells, using [KO Validated] SND1 Rabbit pAb (A5874) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] SND1 Rabbit pAb (A5874)

Western blot analysis of lysates from wild type (WT) and SND1 knockout (KO) HeLa cells, using [KO Validated] SND1 Rabbit pAb (A5874) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit pAb (A5874)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] SND1 Rabbit pAb (A5874) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit pAb (A5874)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] SND1 Rabbit pAb (A5874) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit pAb (A5874)

Immunohistochemistry analysis of paraffin-embedded Rat colon using [KO Validated] SND1 Rabbit pAb (A5874) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit pAb (A5874)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using [KO Validated] SND1 Rabbit pAb (A5874) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] SND1 Rabbit pAb (A5874)

Immunofluorescence analysis of C6 cells using SND1 Rabbit pAb (A5874) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] SND1 Rabbit pAb (A5874)

Immunofluorescence analysis of L929 cells using SND1 Rabbit pAb (A5874) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] SND1 Rabbit pAb (A5874)

Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 3 μg SND1 antibody (A5874). Western blot was performed from the immunoprecipitate using SND1 antibody (A5874) at a dilution of 1:1000.

Overview

Product name[KO Validated] SND1 Rabbit pAb
Catalog No.A5874
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcriptional co-activator that interacts with the acidic domain of Epstein-Barr virus nuclear antigen 2 (EBNA 2), a transcriptional activator that is required for B-lymphocyte transformation. Other transcription factors that interact with this protein are signal transducers and activators of transcription, STATs. This protein is also thought to be essential for normal cell growth. A similar protein in mammals and other organisms is a component of the RNA-induced silencing complex (RISC).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 26-285 of human SND1 (NP_055205.2).
SequenceMVLSGCAIIVRGQPRGGPPPERQINLSNIRAGNLARRAAATQPDAKDTPDEPWAFPAREFLRKKLIGKEVCFTIENKTPQGREYGMIYLGKDTNGENIAESLVAEGLATRREGMRANNPEQNRLSECEEQAKAAKKGMWSEGNGSHTIRDLKYTIENPRHFVDSHHQKPVNAIIEHVRDGSVVRALLLPDYYLVTVMLSGIKCPTFRREADGSETPEPFAAEAKFFTESRLLQRDVQIILESCHNQNILGTILHPNGNIT
Gene ID
Swiss Prot
Synonymsp100; TDRD11; Tudor-SN; D1
Calculated MW102kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, Jurkat, HL-60, MCF7, 293T, Mouse liver, Rat liver
Cellular locationCytoplasm, Melanosome, Nucleus.
Customer validation

WB(Homo sapiens, Mus musculus)

Co-IP(Mus musculus)

IHC(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KO Validated] SND1 Rabbit pAb (A5874)}

Western blot - [KO Validated] SND1 Rabbit pAb (A5874)

Western blot analysis of various lysates using [KO Validated] SND1 Rabbit pAb (A5874) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] SND1 Rabbit pAb (A5874)}

Western blot - [KO Validated] SND1 Rabbit pAb (A5874)

Western blot analysis of various lysates using [KO Validated] SND1 Rabbit pAb (A5874) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] SND1 Rabbit pAb (A5874)}

Western blot - [KO Validated] SND1 Rabbit pAb (A5874)

Western blot analysis of various lysates, using SND1 Rabbit pAb (A5874) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] SND1 Rabbit pAb (A5874)}

Western blot - [KO Validated] SND1 Rabbit pAb (A5874)

Western blot analysis of lysates from wild type (WT) and SND1 knockout (KO) HeLa cells, using [KO Validated] SND1 Rabbit pAb (A5874) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] SND1 Rabbit pAb (A5874)}

Western blot - [KO Validated] SND1 Rabbit pAb (A5874)

Western blot analysis of lysates from wild type (WT) and SND1 knockout (KO) HeLa cells, using [KO Validated] SND1 Rabbit pAb (A5874) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit pAb (A5874)}

Immunohistochemistry - [KO Validated] SND1 Rabbit pAb (A5874)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] SND1 Rabbit pAb (A5874) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit pAb (A5874)}

Immunohistochemistry - [KO Validated] SND1 Rabbit pAb (A5874)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] SND1 Rabbit pAb (A5874) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit pAb (A5874)}

Immunohistochemistry - [KO Validated] SND1 Rabbit pAb (A5874)

Immunohistochemistry analysis of paraffin-embedded Rat colon using [KO Validated] SND1 Rabbit pAb (A5874) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit pAb (A5874)}

Immunohistochemistry - [KO Validated] SND1 Rabbit pAb (A5874)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using [KO Validated] SND1 Rabbit pAb (A5874) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KO Validated] SND1 Rabbit pAb (A5874)}

Immunofluorescence - [KO Validated] SND1 Rabbit pAb (A5874)

Immunofluorescence analysis of C6 cells using SND1 Rabbit pAb (A5874) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] SND1 Rabbit pAb (A5874)}

Immunofluorescence - [KO Validated] SND1 Rabbit pAb (A5874)

Immunofluorescence analysis of L929 cells using SND1 Rabbit pAb (A5874) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] SND1 Rabbit pAb (A5874)}

Immunoprecipitation - [KO Validated] SND1 Rabbit pAb (A5874)

Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 3 μg SND1 antibody (A5874). Western blot was performed from the immunoprecipitate using SND1 antibody (A5874) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A5874? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)