제품 > 항체 > 단일클론항체(mAb)

[KO Validated] STAT3 Rabbit mAb (A19566)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from wild type (WT) and STAT3 knockout (KO) HeLa cells using [KO Validated] STAT3 Rabbit mAb (A19566) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from A549 cells using [KO Validated] STAT3 Rabbit mAb (A19566) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from Mouse liver using [KO Validated] STAT3 Rabbit mAb (A19566) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - [KO Validated] STAT3 Rabbit mAb (A19566)

Immunohistochemistry analysis of paraffin-embedded Human breast tissue using [KO Validated] STAT3 Rabbit mAb (A19566) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KO Validated] STAT3 Rabbit mAb (A19566) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using [KO Validated] STAT3 Rabbit mAb (A19566) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product name	[KO Validated] STAT3 Rabbit mAb
Catalog No.	A19566
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2603

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 600-700 of human STAT3 (P40763).
Sequence	TKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPG
Gene ID
Swiss Prot
Synonyms	APRF; HIES; ADMIO; ADMIO1; T3
Calculated MW	88kDa
Observed MW	79kDa/86kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 IHC-P 1:200 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	A549, HeLa, Mouse liver
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Homo sapiens, Rattus norvegicus, Mus musculus, Staphylococcus aureus, Mus musculus) IF(Homo sapiens) IP(Homo sapiens, Mus musculus) RT-qPCR(Homo sapiens) WB(Homo sapiens) IHC(Homo sapiens) Co-IP(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] STAT3 Rabbit mAb images

Western blot - [KO Validated] STAT3 Rabbit mAb (A19566)

Immunohistochemistry - [KO Validated] STAT3 Rabbit mAb (A19566)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

J Ethnopharmacol

Application:

IF:

4.36

Species:

Homo sapiens

PMID:

33716082

Title:

Taraxacum mongolicum extract inhibited malignant phenotype of triple-negative breast cancer cells in tumor-associated macrophages microenvironment through suppressing IL-10 / STAT3 / PD-L1 signaling pathways

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

JCI insight

Application:

IF:

9.48

Species:

Homo sapiens

PMID:

36166295

Title:

Interleukin-37 protects against acinar cell pyroptosis in acute pancreatitis

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.19

Species:

Rattus norvegicus

PMID:

35427726

Title:

The therapeutic mechanism of PuRenDan for the treatment of diabetic nephropathy: Network pharmacology and experimental verification

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Advanced healthcare materials

Application:

IF:

11.09

Species:

Mus musculus

PMID:

36603196

Title:

Antifibrotic Effects of Tetrahedral Framework Nucleic Acids by Inhibiting Macrophage Polarization and Macrophage‐myofibroblast Transition in Bladder Remodeling

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

iScience

Application:

IF:

6.1

Species:

Staphylococcus aureus

PMID:

36687318

Title:

The Staphylococcus aureus protein IsdA increases SARS CoV-2 replication by modulating JAK-STAT signaling - PMC

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Aging

Application:

IF:

5.95

Species:

Mus musculus

PMID:

37552127

Title:

Inhibition of BACE1 attenuates microglia-induced neuroinflammation after intracerebral hemorrhage by suppressing STAT3 activation - PMC

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Function (Oxford, England)

Application:

IF:

Species:

Mus musculus

PMID:

38020066

Title:

Neutrophil-specific ORAI1 Calcium Channel Inhibition Reduces Pancreatitis-associated Acute Lung Injury - PMC

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

37894738

Title:

Notopterol Suppresses IL-17-Induced Proliferation and Invasion of A549 Lung Adenocarcinoma Cells via Modulation of STAT3, NF-B, and AP-1 Activation - PMC

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

IF:

5.83

Species:

Homo sapiens

PMID:

36468780

Title:

VASN promotes colorectal cancer progression by activating the YAP/TAZ and AKT signaling pathways via YAP

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Mol Immunol

Application:

IF:

4.174

Species:

Homo sapiens

PMID:

34653793

Title:

S100A11 regulates nasal epithelial cell remodeling and inflammation in CRSwNPs via the RAGE-mediated AMPK-STAT3 pathway

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Biochemical pharmacology

Application:

WB, IF

IF:

6.1

Species:

Homo sapiens

PMID:

33744226

Title:

Rabeprazole suppresses cell proliferation in gastric epithelial cells by targeting STAT3-mediated glycolysis

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Frontiers in psychiatry

Application:

IF:

5.435

Species:

Homo sapiens, Mus musculus

PMID:

35509887

Title:

Deciphering Risperidone-Induced Lipogenesis by Network Pharmacology and Molecular Validation

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Journal of translational medicine

Application:

IF:

8.44

Species:

Homo sapiens, Mus musculus

PMID:

36578083

Title:

KPNA2 promotes angiogenesis by regulating STAT3 phosphorylation - PMC

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Journal of immunology (Baltimore, Md. : 1950)

Application:

IF:

5.42

Species:

Mus musculus

PMID:

37459052

Title:

Epithelial TIPE1 Protein Guards against Colitis by Inhibiting TNF-Mediated Inflammation

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

BMC genomics

Application:

IF:

4.54

Species:

Mus musculus

PMID:

38310234

Title:

Identification and analysis of key circRNAs in the mouse embryonic ovary provides insight into primordial follicle development - PMC

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Pharmaceuticals (Basel, Switzerland)

Application:

IF:

5.21

Species:

Homo sapiens

PMID:

36678513

Title:

Preclinical Studies of Chiauranib Show It Inhibits Transformed Follicular Lymphoma through the VEGFR2/ERK/STAT3 Signaling Pathway - PMC

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Cell death & disease

Application:

WB, IP

IF:

Species:

Homo sapiens, Mus musculus

PMID:

38129408

Title:

USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24 - PMC

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

BMC microbiology

Application:

IF:

4.2

Species:

Mus musculus

PMID:

38521894

Title:

Clostridium butyricum Inhibits the Inflammation in Children with Primary Nephrotic Syndrome by Regulating Th17/Tregs Balance via Gut-Kidney Axis

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

iScience

Application:

IF:

5.8

Species:

Mus musculus

PMID:

38559585

Title:

The mutation of NSUN5 R295C promotes preeclampsia by impairing decidualization through downregulating IL-11R

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Journal of inflammation research

Application:

IF:

4.63

Species:

Mus musculus

PMID:

35668915

Title:

Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway - PMC

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Nutrients

Application:

IF:

6.7

Species:

Mus musculus

PMID:

36432480

Title:

Promoting Effect of L-Fucose on the Regeneration of Intestinal Stem Cells through AHR/IL-22 Pathway of Intestinal Lamina Propria Monocytes - PMC

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Cell biology and toxicology

Application:

IF:

6.81

Species:

Homo sapiens

PMID:

36522543

Title:

NSD1 promotes esophageal cancer tumorigenesis via HIF1 signaling

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Cell death & disease

Application:

RT-qPCR, WB

IF:

Species:

Homo sapiens

PMID:

37024465

Title:

CMTR1 promotes colorectal cancer cell growth and immune evasion by transcriptionally regulating STAT3 - PMC

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Journal of translational medicine

Application:

IHC, WB

IF:

7.4

Species:

Homo sapiens

PMID:

38637862

Title:

Short-chain fatty acid-producing bacterial strains attenuate experimental ulcerative colitis by promoting M2 macrophage polarization via JAK/STAT3/FOXO3 axis inactivation - PMC

References for [KO Validated] STAT3 Rabbit mAb (A19566)

Product:

[KO Validated] STAT3 Rabbit mAb

Journal:

Journal of virology

Application:

Co-IP, WB

IF:

5.4

Species:

Homo sapiens

PMID: