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[KO Validated] SUZ12 Rabbit pAb (A7786)

KO/KDValidated

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] SUZ12 Rabbit pAb (A7786)

Western blot analysis of various lysates using [KO Validated] SUZ12 Rabbit pAb (A7786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] SUZ12 Rabbit pAb (A7786)

Western blot analysis of lysates from wild type (WT) and SUZ12 knockout (KO) HeLa cells, using [KO Validated] SUZ12 Rabbit pAb (A7786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - [KO Validated] SUZ12 Rabbit pAb (A7786)

Immunofluorescence analysis of C6 cells using [KO Validated] SUZ12 Rabbit pAb (A7786) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] SUZ12 Rabbit pAb (A7786)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg SUZ12 antibody (A7786). Western blot was performed from the immunoprecipitate using SUZ12 antibody (A7786) at a dilution of 1:500.

Overview

Product name[KO Validated] SUZ12 Rabbit pAb
Catalog No.A7786
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2).
SequenceCHSRFIFNYVYHPKGARIDVSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL
Gene ID
Swiss Prot
SynonymsCHET9; IMMAS; JJAZ1; 12
Calculated MW83kDa
Observed MW83kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, BT-474, MCF-7, 293T
Cellular locationNucleus
Customer validation

IP(Homo sapiens)

WB(Homo sapiens)

IP(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] SUZ12 Rabbit pAb (A7786)}

Western blot - [KO Validated] SUZ12 Rabbit pAb (A7786)

Western blot analysis of various lysates using [KO Validated] SUZ12 Rabbit pAb (A7786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] SUZ12 Rabbit pAb (A7786)}

Western blot - [KO Validated] SUZ12 Rabbit pAb (A7786)

Western blot analysis of lysates from wild type (WT) and SUZ12 knockout (KO) HeLa cells, using [KO Validated] SUZ12 Rabbit pAb (A7786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - [KO Validated] SUZ12 Rabbit pAb (A7786)}

Immunofluorescence - [KO Validated] SUZ12 Rabbit pAb (A7786)

Immunofluorescence analysis of C6 cells using [KO Validated] SUZ12 Rabbit pAb (A7786) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] SUZ12 Rabbit pAb (A7786)}

Immunoprecipitation - [KO Validated] SUZ12 Rabbit pAb (A7786)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg SUZ12 antibody (A7786). Western blot was performed from the immunoprecipitate using SUZ12 antibody (A7786) at a dilution of 1:500.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A7786? Please let us know so that we can cite the reference in this datasheet.