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[KO Validated] Smad3 Rabbit mAb (A19115)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using Smad3 Rabbit mAb (A19115) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from Rat testis, using Smad3 Rabbit mAb (A19115) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from wild type(WT) and Smad3 Rabbit mAb knockout (KO) HCT116(KO) cells, using Smad3 Rabbit mAb (A19115) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunohistochemistry analysis of paraffin-embedded Human testis using [KO Validated] Smad3 Rabbit mAb (A19115) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] Smad3 Rabbit mAb (A19115) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	[KO Validated] Smad3 Rabbit mAb
Catalog No.	A19115
Host species	Rabbit
Purification method	Protein A
Isotype	IgG
CloneNo.	ARC53861

Background

The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Smad3 (NP_005893.1).
Sequence	HHELRAMELCEFAFNMKKDEVCVNPYHYQRVETPVLPPVLVPRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSM
Gene ID
Swiss Prot
Synonyms	LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436; d3
Calculated MW	48kDa
Observed MW	52kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:20000 - 1:80000 IHC-P 1:200 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	HCT116, A-549, C2C12, Rat testis
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Mus musculus, Rattus norvegicus, Homo sapiens) IHC(Homo sapiens, Mus musculus) IP(Homo sapiens) IF(Mus musculus ) IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] Smad3 Rabbit mAb images

Western blot - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunohistochemistry - [KO Validated] Smad3 Rabbit mAb (A19115)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

J Ethnopharmacol

Application:

IF:

4.36

Species:

Mus musculus

PMID:

32783983

Title:

Baihe Wuyao decoction ameliorates CCl4-induced chronic liver injury and liver fibrosis in mice through blocking TGF-β1/Smad2/3 signaling, anti-inflammation and anti-oxidation effects

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Food Chem Toxicol

Application:

IF:

6.02

Species:

Mus musculus

PMID:

34600025

Title:

Prenatal EGCG exposure-induced heart mass reduction in adult male mice and underlying mechanisms

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Front Immunol

Application:

IF:

7.561

Species:

Mus musculus

PMID:

34868004

Title:

Epigenetic Modulation of Class-Switch DNA Recombination to IgA by miR-146a Through Downregulation of Smad2, Smad3 and Smad4

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Current molecular medicine

Application:

IF:

2.61

Species:

Mus musculus

PMID:

29879887

Title:

Hesperetin mitigates bile duct ligation-induced liver fibrosis by inhibiting extracellular matrix and cell apoptosis via the TGF-β1/Smad pathway

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Experimental animals

Application:

IF:

1.79

Species:

Mus musculus

PMID:

34615811

Title:

Suppression of ADAM8 attenuates angiotensin II-induced cardiac fibrosis and endothelial-mesenchymal transition via inhibiting TGF-β1/Smad2/Smad3 pathways

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

The Journal of clinical investigation

Application:

IF:

19.45

Species:

Mus musculus

PMID:

35085106

Title:

Smad3 methylation by EZH2 promotes its activation and tumor metastasis

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

EBioMedicine

Application:

IF:

11.2

Species:

Mus musculus

PMID:

36182775

Title:

Inhibition of HIPK2 protects stress-induced pathological cardiac remodeling - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

5.57

Species:

Mus musculus

PMID:

35358666

Title:

Respiratory exposure to graphene quantum dots causes fibrotic effects on lung, liver and kidney of mice

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Cells

Application:

IF:

7.66

Species:

Mus musculus

PMID:

36497076

Title:

Follistatin Is a Novel Chemoattractant for Migration and Invasion of Placental Trophoblasts of Mice - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Inflammation

Application:

IF:

4.65

Species:

Mus musculus

PMID:

36577924

Title:

IRAK-M Regulates Proliferative and Invasive Phenotypes of Lung Fibroblasts

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

BMC microbiology

Application:

IF:

4.46

Species:

Mus musculus

PMID:

36949381

Title:

Improvement of vaginal probiotics Lactobacillus crispatus on intrauterine adhesion in mice model and in clinical practice - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

PeerJ

Application:

IF:

3.06

Species:

Rattus norvegicus

PMID:

37786576

Title:

Rivaroxaban, a direct inhibitor of coagulation factor Xa, attenuates adverse cardiac remodeling in rats by regulating the PAR-2 and TGF-1 signaling pathways - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

PeerJ

Application:

IF:

3.06

Species:

Mus musculus

PMID:

37790634

Title:

Loss of Farnesoid X receptor (FXR) accelerates dysregulated glucose and renal injury in db/db mice - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Scientific reports

Application:

IF:

4.99

Species:

Homo sapiens

PMID:

37596361

Title:

Inhibitory effects of Schisandrin C on collagen behavior in pulmonary fibrosis - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Molecular cell

Application:

IF:

19.32

Species:

Homo sapiens

PMID:

37935198

Title:

The proteasome component PSMD14 drives myelomagenesis through a histone deubiquitinase activity

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

WB, IHC

IF:

7.41

Species:

Homo sapiens

PMID:

36068784

Title:

Losartan ameliorates renal interstitial fibrosis through metabolic pathway and Smurfs-TGF-/Smad

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Scientific reports

Application:

IF:

4.99

Species:

Homo sapiens

PMID:

38071381

Title:

PNO1 promotes the progression of osteosarcoma via TGF- and YAP/TAZ pathway - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Molecular biology reports

Application:

IF:

2.74

Species:

Rattus norvegicus

PMID:

38158445

Title:

Muscone inhibits angiotensin II-induced cardiac hypertrophy through the STAT3, MAPK and TGF-/SMAD signaling pathways - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Cancer biology & therapy

Application:

IF:

4.87

Species:

Homo sapiens

PMID:

37981873

Title:

miRNA-660-3p inhibits malignancy in glioblastoma via negative regulation of APOC1-TGF2 signaling pathway

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Cancer biology & therapy

Application:

IF:

4.87

Species:

Homo sapiens

PMID:

37981873

Title:

miRNA-660-3p inhibits malignancy in glioblastoma via negative regulation of APOC1-TGF2 signaling pathway

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.19

Species:

Mus musculus

PMID:

38171465

Title:

Bletilla striata polysaccharide attenuated the progression of pulmonary fibrosis by inhibiting TGF-1/Smad signaling pathway

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Oncogene

Application:

IF:

8.75

Species:

Homo sapiens

PMID:

36991117

Title:

PRMT5 methylating Smad4 activates TGF- signaling and promotes colorectal cancer metastasis

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Renal failure

Application:

IF:

3.22

Species:

Rattus norvegicus

PMID:

38357745

Title:

Study on effects and relevant mechanisms of Mudan granules on renal fibrosis in streptozotocin-induced diabetes rats

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

7.9

Species:

Mus musculus

PMID:

38452697

Title:

Paris polyphylla ethanol extract and polyphyllin I ameliorate adenomyosis by inhibiting epithelialmesenchymal transition

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

IF:

5.7

Species:

Homo sapiens,Mus musculus

PMID:

38424494

Title:

USF1 transcriptionally activates USP14 to drive atherosclerosis by promoting EndMT through NLRC5/Smad2/3 axis - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Cells

Application:

IF:

7.666

Species:

Mus musculus

PMID:

34440873

Title:

miR-135a Suppresses Granulosa Cell Growth by Targeting Tgfbr1 and Ccnd2 during Folliculogenesis in Mice

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Mus musculus

PMID:

35721120

Title:

Shenkang Injection for Treating Renal Fibrosis-Metabonomics and Regulation of E3 Ubiquitin Ligase Smurfs on TGF-/Smads Signal Transduction - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Mus musculus

PMID:

35721120

Title:

Shenkang Injection for Treating Renal Fibrosis-Metabonomics and Regulation of E3 Ubiquitin Ligase Smurfs on TGF-/Smads Signal Transduction - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Nature communications

Application:

WB, IP

IF:

17.69

Species:

Homo sapiens

PMID:

35915084

Title:

Breast cancer cell-derived extracellular vesicles promote CD8+T cell exhaustion via TGF- type II receptor signaling - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Molecular carcinogenesis

Application:

WB, IHC

IF:

5.13

Species:

Mus musculus

PMID:

37378449

Title:

Activin A/ACVR2A axis inhibits epithelialtomesenchymal transition in colon cancer by activating SMAD2

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Theranostics

Application:

WB, IF

IF:

11.6

Species:

Mus musculus

PMID:

37441592

Title:

Tsp-1+ microglia attenuate retinal neovascularization by maintaining the expression of Smad3 in endothelial cells through exosomes with decreased miR-27a-5p - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

eLife

Application:

IF:

8.71

Species:

Homo sapiens,Mus musculus

PMID:

35975983

Title:

Biphasic regulation of osteoblast development via the ERK MAPK-mTOR pathway - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Cancer cell international

Application:

IF:

5.8

Species:

Homo sapiens

PMID:

38769521

Title:

Smad2/3/4 complex could undergo liquid liquid phase separation and induce apoptosis through TAT in hepatocellular carcinoma - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

7.9

Species:

Mus musculus

PMID:

38833791

Title:

Targeting antioxidant factor Nrf2 by raffinose ameliorates lipid dysmetabolism-induced pyroptosis, inflammation and fibrosis in NAFLD

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

IF:

5.7

Species:

Mus musculus

PMID:

38982366

Title:

Deletion of GPR81 activates CREB/Smad7 pathway and alleviates liver fibrosis in mice - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Renal failure

Application:

IF:

Species:

Homo sapiens,Mus musculus

PMID:

38952291

Title:

Chlorogenic acid alleviates renal fibrosis by reducing lipid accumulation in diabetic kidney disease through suppressing the Notch1 and Stat3 signaling pathway - PMC

References for [KO Validated] Smad3 Rabbit mAb (A19115)

Product:

[KO Validated] Smad3 Rabbit mAb

Journal:

Journal of hazardous materials

Application:

IF:

13.6

Species:

Mus musculus

PMID:

38852254