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[KO Validated] c-Myc Rabbit mAb (A19032)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

UT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ K562 cells with 1μg c-Myc Rabbit mAb(A19032), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of ESE1 in representative gene loci (NPM1), as shown in figure.

ABclonal:Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot analysis of various lysates using [KO Validated] c-Myc Rabbit mAb (A19032) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from wild type (WT) and c-Myc knockout (KO) HCT 116 cells, using [KO Validated] c-Myc Rabbit mAb (A19032) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Chromatin Immunoprecipitation - [KO Validated] c-Myc Rabbit mAb (A19032)

Chromatin immunoprecipitation analysis of extracts of K562 cells, using c-Myc antibody (A19032) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review

Overview

Product name	[KO Validated] c-Myc Rabbit mAb
Catalog No.	A19032
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0412

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human c-Myc (P01106).
Sequence	MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLE
Gene ID
Swiss Prot
Synonyms	MRTL; MYCC; c-Myc; bHLHe39; yc
Calculated MW	51kDa
Observed MW	50-60kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP 5μg antibody for 10μg-15μg of Chromatin CUT&Tag 10⁵ cells /1 μg
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Jurkat, MCF7, HCT 116
Cellular location	Nucleus, nucleolus, nucleoplasm.
Customer validation	(293T MOLT4 IGROV-1) WB(Homo sapiens, Mus musculus, Cyprinus carpio, Mus musculus) IP(Homo sapiens) ChIP(Arabidopsis thaliana, Homo sapiens, Mus musculus) qPCR(Homo sapiens) Co-IP(Homo sapiens) WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] c-Myc Rabbit mAb images

- [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Chromatin Immunoprecipitation - [KO Validated] c-Myc Rabbit mAb (A19032)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

Front Oncol

Application:

IF:

6.244

Species:

Homo sapiens

PMID:

33552953

Title:

Rhophilin-2 Upregulates Glutamine Synthetase by Stabilizing c-Myc Protein and Confers Resistance to Glutamine Deprivation in Lung Cancer

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

Cancer Cell Int

Application:

IF:

5.72

Species:

Mus musculus

PMID:

33858423

Title:

Trichostatin A downregulates bromodomain and extra-terminal proteins to suppress osimertinib resistant non-small cell lung carcinoma

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

Frontiers in oncology

Application:

IF:

5.73

Species:

Mus musculus

PMID:

35992875

Title:

Novel myeloma patient-derived xenograft models unveil the potency of anlotinib to overcome bortezomib resistance - PMC

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

BioMed research international

Application:

IF:

Species:

Homo sapiens

PMID:

36158888

Title:

CTGF Promotes the Osteoblast Differentiation of Human Periodontal Ligament Stem Cells by Positively Regulating BMP2/Smad Signal Transduction - PMC

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

Fish & shellfish immunology

Application:

IF:

4.62

Species:

Cyprinus carpio

PMID:

36496140

Title:

Koumine induces apoptosis in Cyprinus carpio liver cells by regulating JAK-STAT and p53 signaling pathways

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

Heliyon

Application:

IF:

3.77

Species:

Homo sapiens

PMID:

38046159

Title:

SOX8 promotes tumor growth and metastasis through FZD6-dependent Wnt/-catenin signaling in colorectal carcinoma - PMC

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

Chemosphere

Application:

IF:

8.8

Species:

Homo sapiens

PMID:

37742764

Title:

Zinc attenuates sulfamethoxazole-induced lipotoxicity by reversing sulfamethoxazole-induced mitochondrial dysfunction and lysosome impairment in a freshwater teleost

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

Medical oncology (Northwood, London, England)

Application:

IF:

3.73

Species:

Homo sapiens, Mus musculus

PMID:

37634215

Title:

EZH2 inhibitor Tazemetostat synergizes with JQ-1 in esophageal cancer by inhibiting c-Myc signaling pathway

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

Plant molecular biology

Application:

ChIP

IF:

4.33

Species:

Arabidopsis thaliana

PMID:

37973764

Title:

GmWRKY17-mediated transcriptional regulation of GmDREB1D and GmABA2 controls drought tolerance in soybean

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

Cancer research communications

Application:

ChIP, qPCR

IF:

Species:

Homo sapiens

PMID:

38284882

Title:

HDAC Inhibition Induces CD26 Expression on Multiple Myeloma Cells via the c-Myc/Sp1-mediated Promoter Activation - PMC

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

iScience

Application:

Co-IP, WB

IF:

6.1

Species:

Homo sapiens

PMID:

38161423

Title:

A helicase-independent role of DHX15 promotes MYC stability and acute leukemia cell survival - PMC

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

Nature cell biology

Application:

ChIP

IF:

21.3

Species:

Mus musculus

PMID:

38321204

Title:

SHP-1 inhibition targets leukaemia stem cells to restore immunosurveillance and enhance chemosensitivity by metabolic reprogramming

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Homo sapiens

PMID:

37290325

Title:

Aspirin induces immunogenic cell death and enhances cancer immunotherapy in colorectal cancer

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

The journals of gerontology. Series A, Biological sciences and medical sciences

Application:

IF:

5.1

Species:

Homo sapiens

PMID:

38407305

Title:

DHCR24 insufficiency promotes vascular endothelial cell senescence and endothelial dysfunction via inhibition of Caveolin-1/ERK signaling

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

Oncogene

Application:

IF:

Species:

Homo sapiens

PMID:

38783100

Title:

tRF-33-P4R8YP9LON4VDP inhibits gastric cancer progression via modulating STAT3 signaling pathway in an AGO2-dependent manner

References for [KO Validated] c-Myc Rabbit mAb (A19032)

Product:

[KO Validated] c-Myc Rabbit mAb

Journal:

Oncology research

Application:

IF:

3.1

Species:

Homo sapiens

PMID:

39220139

Title:

NCAPD2 serves as a potential prognostic biomarker for lung adenocarcinoma and promotes cell proliferation, migration, invasion and cell cycle in vitro - PMC