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Ki67 Rabbit pAb (A11390)

Reviews (2)Publications (46) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunofluorescence - Ki67 Rabbit pAb (A11390)

Immunofluorescence analysis of HeLa cells using Ki67 Rabbit pAb (A11390) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Ki67 Rabbit pAb(A11390)
Review
ABclonal: review for Ki67 Rabbit pAb(A11390)

Overview

Product nameKi67 Rabbit pAb
Catalog No.A11390
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables protein C-terminus binding activity. Involved in regulation of chromosome segregation and regulation of mitotic nuclear division. Located in chromosome; nuclear body; and nucleolus. Colocalizes with condensed chromosome. Implicated in Crohn's disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Biomarker of several diseases, including Barrett's esophagus; autoimmune disease of musculoskeletal system (multiple); endocrine gland cancer (multiple); gastrointestinal system cancer (multiple); and interstitial cystitis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 700-800 of human Ki67 (NP_002408.3).
SequenceGEVHSQFSTGHANSPCTIIIGKAHTEKVHVPARPYRVLNNFISNQKMDFKEDLSGIAEMFKTPVKEQPQLTSTCHIAISNSENLLGKQFQGTDSGEEPLLP
Gene ID
Swiss Prot
SynonymsKIA; MIB-; MIB-1; PPP1R105; Ki67
Calculated MW359kDa
Observed MW359kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    
Positive samples22Rv1, Raji
Cellular locationChromosome, Nucleus, nucleolus
Customer validation

(Mouse tissue, Mouse colon tissue)

WB(Homo sapiens, Mus musculus)

IF(Rattus norvegicus, Macaca mulatta, Homo sapiens, Mus musculus, Ovis aries, Mus musculus)

IHC(Mus musculus, Homo sapiens)

IHC(Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus)

IHC(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Immunofluorescence - Ki67 Rabbit pAb (A11390)}

Immunofluorescence - Ki67 Rabbit pAb (A11390)

Immunofluorescence analysis of HeLa cells using Ki67 Rabbit pAb (A11390) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (8)

Secondary Antibodies (26)