Description
Klenow Fragment (3'→ 5' exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5'→ 3' exonuclease activity and has mutations (D355A, E357A) which abolish the 3'→ 5' exonuclease activity. Klenow Fragment (3'→ 5' exo-) is isolated from a recombinant source. It generates probes using random primers and shows moderate strand displacement activity. It can be used in random primer labeling, DNA sequencing by the Sanger dideoxy method, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols.
Workflow Chart
Storage
-20°C
Product Components
| Components | Concentration | 200 U | 1, 000 U | 5, 000 U |
| Klenow Fragment 3’→5’ exo- | 5, 000 U/mL | 40 μL | 200 μL | 1 mL |
| 10X ABuffer B | 10X | 1.25 mL | 1.25 mL | 1.25 mL X 4 |
Documents
###RM20516|||10X ABuffer B###RM20126)
