Western blot - LDHA Rabbit pAb (A1146)
Western blot analysis of lysates from HeLa cells, using LDHA Rabbit pAb (A1146) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot - LDHA Rabbit pAb (A1146)
Western blot analysis of various lysates, using [KD Validated] LDHA Rabbit pAb (A1146) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot - LDHA Rabbit pAb (A1146)
Western blot analysis of lysates from wild type (WT) and LDHA knockdown (KD) 293T cells using LDHA Rabbit pAb (A1146) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot - LDHA Rabbit pAb (A1146)
Western blot analysis of lysates from Rat liver using LDHA Rabbit pAb (A1146) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot - LDHA Rabbit pAb (A1146)
Western blot analysis of lysates from wild type (WT) and LDHA knockdown (KD) 293T cells using LDHA Rabbit pAb (A1146) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry - LDHA Rabbit pAb (A1146)
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - LDHA Rabbit pAb (A1146)
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - LDHA Rabbit pAb (A1146)
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - LDHA Rabbit pAb (A1146)
Immunohistochemistry analysis of paraffin-embedded Rat large intestine tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - LDHA Rabbit pAb (A1146)
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - LDHA Rabbit pAb (A1146)
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - LDHA Rabbit pAb (A1146)
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence - LDHA Rabbit pAb (A1146)
Immunofluorescence analysis of HeLa cells using LDHA Rabbit pAb (A1146) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - LDHA Rabbit pAb (A1146)
Immunofluorescence analysis of MCF7 cells using LDHA Rabbit pAb (A1146) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.