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LDHA Rabbit pAb (A1146)

Reviews (3)Publications (34) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LDHA Rabbit pAb (A1146)

Western blot analysis of lysates from HeLa cells, using LDHA Rabbit pAb (A1146) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - LDHA Rabbit pAb (A1146)

Western blot analysis of various lysates, using [KD Validated] LDHA Rabbit pAb (A1146) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - LDHA Rabbit pAb (A1146)

Western blot analysis of lysates from wild type (WT) and LDHA knockdown (KD) 293T cells using LDHA Rabbit pAb (A1146) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - LDHA Rabbit pAb (A1146)

Western blot analysis of lysates from Rat liver using LDHA Rabbit pAb (A1146) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - LDHA Rabbit pAb (A1146)

Western blot analysis of lysates from wild type (WT) and LDHA knockdown (KD) 293T cells using LDHA Rabbit pAb (A1146) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Rat large intestine tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - LDHA Rabbit pAb (A1146)

Immunofluorescence analysis of HeLa cells using LDHA Rabbit pAb (A1146) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LDHA Rabbit pAb (A1146)

Immunofluorescence analysis of MCF7 cells using LDHA Rabbit pAb (A1146) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for LDHA Rabbit pAb(A1146)
Review
ABclonal: review for LDHA Rabbit pAb(A1146)
Review
ABclonal: review for LDHA Rabbit pAb(A1146)

Overview

Product nameLDHA Rabbit pAb
Catalog No.A1146
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-332 of human LDHA (NP_005557.1).
SequenceMATLKDQLIYNLLKEEQTPQNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLRTPKIVSGKDYNVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPNCKLLIVSNPVDILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVWSGMNVAGVSLKTLHPDLGTDKDKEQWKEVHKQVVESAYEVIKLKGYTSWAIGLSVADLAESIMKNLRRVHPVSTMIKGLYGIKDDVFLSVPCILGQNGISDLVKVTLTSEEEARLKKSADTLWGIQKELQF
Gene ID
Swiss Prot
SynonymsLDHM; GSD11; PIG19; HEL-S-133P; LDHA
Calculated MW37kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T, Rat liver, HeLa
Cellular locationCytoplasm.
Customer validation

(Human lung cancer cells, unknow)

WB(Mus musculus, Homo sapiens, Sus scrofa, Gallus gallus, Mus musculus)

IP(Homo sapiens)

IHC(Homo sapiens)

IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - LDHA Rabbit pAb (A1146)}

Western blot - LDHA Rabbit pAb (A1146)

Western blot analysis of lysates from HeLa cells, using LDHA Rabbit pAb (A1146) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - LDHA Rabbit pAb (A1146)}

Western blot - LDHA Rabbit pAb (A1146)

Western blot analysis of various lysates, using [KD Validated] LDHA Rabbit pAb (A1146) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - LDHA Rabbit pAb (A1146)}

Western blot - LDHA Rabbit pAb (A1146)

Western blot analysis of lysates from wild type (WT) and LDHA knockdown (KD) 293T cells using LDHA Rabbit pAb (A1146) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - LDHA Rabbit pAb (A1146)}

Western blot - LDHA Rabbit pAb (A1146)

Western blot analysis of lysates from Rat liver using LDHA Rabbit pAb (A1146) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - LDHA Rabbit pAb (A1146)}

Western blot - LDHA Rabbit pAb (A1146)

Western blot analysis of lysates from wild type (WT) and LDHA knockdown (KD) 293T cells using LDHA Rabbit pAb (A1146) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)}

Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)}

Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)}

Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)}

Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Rat large intestine tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)}

Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)}

Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - LDHA Rabbit pAb (A1146)}

Immunohistochemistry - LDHA Rabbit pAb (A1146)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using LDHA Rabbit pAb (A1146) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - LDHA Rabbit pAb (A1146)}

Immunofluorescence - LDHA Rabbit pAb (A1146)

Immunofluorescence analysis of HeLa cells using LDHA Rabbit pAb (A1146) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - LDHA Rabbit pAb (A1146)}

Immunofluorescence - LDHA Rabbit pAb (A1146)

Immunofluorescence analysis of MCF7 cells using LDHA Rabbit pAb (A1146) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (4)

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Secondary Antibodies (26)