제품 > 항체 > 단일클론항체(mAb)

LEF1 Rabbit mAb (A4473)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - LEF1 Rabbit mAb (A4473)

Western blot analysis of various lysates using LEF1 Rabbit mAb (A4473) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product nameLEF1 Rabbit mAb
Catalog No.A4473
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1019
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant protein fo a sequence within amino acids 50-150 of human LEF1 (Q9UJU2).
SequenceLADIKSSLVNESEIIPASNGHEVARQAQTSQEPYHDKAREHPDDGKHPDGGLYNKGPSYSSYSGYIMMPNMNNDPYMSNGSLSPPIPRTSNKVPVVQPSHA
Gene ID
Swiss Prot
SynonymsLEF-1; TCF10; TCF7L3; TCF1ALPHA; LEF1
Calculated MW44kDa
Observed MW30-50kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat, Mouse thymus
Cellular locationNucleus
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - LEF1 Rabbit mAb (A4473)}

Western blot - LEF1 Rabbit mAb (A4473)

Western blot analysis of various lysates using LEF1 Rabbit mAb (A4473) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A4473? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)