제품 > 항체 > 다중클론항체(pAb)

LKB1 Rabbit pAb (A2122)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using LKB1 Rabbit pAb (A2122) at 1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from Mouse heart, using LKB1 Rabbit pAb (A2122) at 1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of various lysates, using [KO Validated] LKB1 Rabbit pAb (A2122) at 1:7000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - LKB1 Rabbit pAb (A2122)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using LKB1 Rabbit pAb (A2122) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - LKB1 Rabbit pAb (A2122)

Immunofluorescence analysis of NIH/3T3 cells using LKB1 Rabbit pAb (A2122) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using LKB1 Rabbit pAb (A2122) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using LKB1 Rabbit pAb (A2122) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	LKB1 Rabbit pAb
Catalog No.	A2122
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cell polarity and energy metabolism and functions as a tumor suppressor. Mutations in this gene have been associated with the autosomal dominant Peutz-Jeghers syndrome, as well as with skin, pancreatic, and testicular cancers.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 134-433 of human LKB1 (NP_000446.1).
Sequence	CGMQEMLDSVPEKRFPVCQAHGYFCQLIDGLEYLHSQGIVHKDIKPGNLLLTTGGTLKISDLGVAEALHPFAADDTCRTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLFENIGKGSYAIPGDCGPPLSDLLKGMLEYEPAKRFSIRQIRQHSWFRKKHPPAEAPVPIPPSPDTKDRWRSMTVVPYLEDLHGADEDEDLFDIEDDIIYTQDFTVPGQVPEEEASHNGQRRGLPKAVCMNGTEAAQLSTKSRAEGRAPNPARKACSASSKIRRLSACKQQ
Gene ID
Swiss Prot
Synonyms	PJS; LKB1; hLKB1
Calculated MW	49kDa
Observed MW	49-60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	MCF7, 293T, Mouse heart, Rat heart
Cellular location	Cytoplasm, Membrane, Mitochondrion, Nucleus.
Customer validation	(HeLa, Human,Mouse,Rat) WB(Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa, Capra hircus) IF(Mus musculus) IHC(Mus musculus, Ctenopharyngodon idellus) PCR(Homo sapiens) WB(Ctenopharyngodon idellus)

Documents

Certificate of Compliance

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Lot number

Research Area

LKB1 Rabbit pAb images

Western blot - LKB1 Rabbit pAb (A2122)

Immunohistochemistry - LKB1 Rabbit pAb (A2122)

Immunofluorescence - LKB1 Rabbit pAb (A2122)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A2122

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Reviews List

Product Protocols

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

Biochemical Pharmacology

Application:

IF:

5.858

Species:

Homo sapiens

PMID:

27816546

Title:

Anhydroicaritin improves diet-induced obesity and hyperlipidemia and alleviates insulin resistance by suppressing SREBPs activation

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

Molecular metabolism

Application:

IF:

7.422

Species:

Mus musculus

PMID:

28123944

Title:

Celastrol ameliorates liver metabolic damage caused by a high-fat diet through Sirt1

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

Oncology

Application:

IF:

2.935

Species:

Homo sapiens

PMID:

29566977

Title:

CO suppresses prostate cancer cell growth by directly targeting LKB1/AMPK/mTOR pathway in vitro and in vivo

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

Pathol Res Pract

Application:

IF:

3.25

Species:

Homo sapiens

PMID:

32827803

Title:

Higher expression of miR-150-5p promotes tumorigenesis by suppressing LKB1 in non-small cell lung cancer

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

Phytomedicine

Application:

IF:

5.34

Species:

Rattus norvegicus

PMID:

34333328

Title:

Berberine improves ovulation and endometrial receptivity in polycystic ovary syndrome

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

Eur J Pharmacol

Application:

IF:

4.432

Species:

Mus musculus

PMID:

34224699

Title:

Dapagliflozin attenuates steatosis in livers of high-fat diet-induced mice and oleic acid-treated L02 cells via regulating AMPK/mTOR pathway

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

Diabetes Metab Syndr Obes

Application:

IF:

3.168

Species:

Mus musculus

PMID:

34629883

Title:

Ethyl 2-[2,3,4-Trimethoxy-6-(1-Octanoyl)Phenyl] Acetate (TMPA) Ameliorates Lipid Accumulation by Disturbing the Combination of LKB1 with Nur77 and Activating the AMPK Pathway in HepG2 Cells and Mice Primary Hepatocytes

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

Virulence

Application:

IF:

5.42

Species:

Sus scrofa

PMID:

36694280

Title:

Resistin secreted by porcine alveolar macrophages leads to endothelial cell dysfunction during Haemophilus parasuis infection - PMC

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

Frontiers in oncology

Application:

IHC

IF:

5.73

Species:

Mus musculus

PMID:

36937390

Title:

Gradual deterioration of fatty liver disease to liver cancer via inhibition of AMPK signaling pathways involved in energy-dependent disorders, cellular aging, and chronic inflammation - PMC

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

Journal of neurophysiology

Application:

IF:

2.97

Species:

Rattus norvegicus

PMID:

37042554

Title:

Dexmedetomidine mitigates neuroinflammation and improves early postoperative neurocognitive dysfunction in rats by enhancing autophagy

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

Cells

Application:

IF:

7.66

Species:

Capra hircus

PMID:

37408227

Title:

APN Expression in Serum and Corpus Luteum: Regulation of Luteal Steroidogenesis Is Possibly Dependent on the AdipoR2/AMPK Pathway in Goats

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.92

Species:

Mus musculus

PMID:

36985786

Title:

Ethanol Extract of Rosa laevigata Michx. Fruit Inhibits Inflammatory Responses through NF-B/MAPK Signaling Pathways via AMPK Activation in RAW 264.7 Macrophages - PMC

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

36614295

Title:

Canagliflozin Attenuates Lipotoxicity in Cardiomyocytes by Inhibiting Inflammation and Ferroptosis through Activating AMPK Pathway - PMC

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

International journal of molecular medicine

Application:

PCR

IF:

5.4

Species:

Homo sapiens

PMID:

38963051

Title:

Salusin alleviates lipid metabolism disorders via regulation of the downstream lipogenesis genes through the LKB1/AMPK pathway

References for LKB1 Rabbit pAb (A2122)

Product:

LKB1 Rabbit pAb

Journal:

Journal of animal science and biotechnology

Application:

IHC, WB

IF:

Species:

Ctenopharyngodon idellus

PMID:

39218924

Title:

Methionine deficiency inhibited pyroptosis in primary hepatocytes of grass carp (Ctenopharyngodon idella): possibly via activating the ROS-AMPK-autophagy axis - PMC