제품 > 항체 > 다중클론항체(pAb)

LONP1 Rabbit pAb (A4293)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using LONP1 Rabbit pAb (A4293) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - LONP1 Rabbit pAb (A4293)

Immunohistochemistry analysis of paraffin-embedded rat kidney using LONP1 Rabbit pAb (A4293) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse kidney using LONP1 Rabbit pAb (A4293) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - LONP1 Rabbit pAb (A4293)

Immunoprecipitation analysis of 300 μg extracts of A-549 cells using 3 μg LONP1 antibody (A4293). Western blot was performed from the immunoprecipitate using LONP1 (A4293) at a dilution of 1:1000.

Overview

Product name	LONP1 Rabbit pAb
Catalog No.	A4293
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a mitochondrial matrix protein that belongs to the Lon family of ATP-dependent proteases. This protein mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides in the mitochondrial matrix. It may also have a chaperone function in the assembly of inner membrane protein complexes, and participate in the regulation of mitochondrial gene expression and maintenance of the integrity of the mitochondrial genome. Decreased expression of this gene has been noted in a patient with hereditary spastic paraplegia (PMID:18378094). Alternatively spliced transcript variants have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 120-400 of human LONP1 (NP_004784.2).
Sequence	VFPHLPLIAITRNPVFPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQL
Gene ID
Swiss Prot
Synonyms	LON; LONP; PIM1; hLON; LonHS; CODASS; PRSS15; LONP1
Calculated MW	106kDa
Observed MW	106kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	A-549, HL-60, LO2, HT-1080, BT-474, Mouse heart, Mouse kidney, Mouse liver, Rat kidney
Cellular location	Mitochondrion matrix
Customer validation	WB(Mus musculus, Homo sapiens, Rattus norvegicus) IF(Rattus norvegicus)

Documents

Certificate of Compliance

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Research Area

LONP1 Rabbit pAb images

Western blot - LONP1 Rabbit pAb (A4293)

Immunohistochemistry - LONP1 Rabbit pAb (A4293)

Immunoprecipitation - LONP1 Rabbit pAb (A4293)

Immunoprecipitation analysis of 300 μg extracts of A-549 cells using 3 μg LONP1 antibody (A4293). Western blot was performed from the immunoprecipitate using LONP1 (A4293) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.