제품 > 항체 > 다중클론항체(pAb)

Lamin A/C Rabbit pAb (A0249)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using Lamin A/C Rabbit pAb (A0249) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Lamin A/C Rabbit pAb (A0249)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Lamin A/C Rabbit pAb (A0249) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain using Lamin A/C Rabbit pAb (A0249) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver using Lamin A/C Rabbit pAb (A0249) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Lamin A/C Rabbit pAb (A0249)

Immunofluorescence analysis of NIH/3T3 cells using Lamin A/C Rabbit pAb (A0249) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	Lamin A/C Rabbit pAb
Catalog No.	A0249
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A/C (NP_733821.1).
Sequence	SSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSS
Gene ID
Swiss Prot
Synonyms	FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B; Lamin A/C
Calculated MW	74kDa
Observed MW	68kDa/72kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP 1:20 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa, NIH/3T3, PC-12
Cellular location	Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm.
Customer validation	(hTERT cells, Primary mouse liver, Human cells, U251, Mouse splenocytes) WB(Homo sapiens, Mus musculus, Sus scrofa, Rattus norvegicus, Mus musculus) IF(Homo sapiens, Chlorocebus aethiops ) IHC(Mus musculus, Homo sapiens) IF(Homo sapiens) ELISA(Mus musculus)

Documents

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Research Area

Lamin A/C Rabbit pAb images

Western blot - Lamin A/C Rabbit pAb (A0249)

Immunohistochemistry - Lamin A/C Rabbit pAb (A0249)

Immunofluorescence - Lamin A/C Rabbit pAb (A0249)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Journal of Immunology

Application:

IF:

5.422

Species:

Homo sapiens

PMID:

26202983

Title:

MicroRNA-15b Modulates Japanese Encephalitis Virus-Mediated Inflammation via Targeting RNF125

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Scientific Reports

Application:

IF:

4.379

Species:

Homo sapiens

PMID:

26931521

Title:

Transcriptional regulation of miR-15b by c-Rel and CREB in Japanese encephalitis virus infection

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Journal Of Proteome Research

Application:

IF:

4.466

Species:

Homo sapiens

PMID:

26485063

Title:

Quantitative Label-Free Phosphoproteomics Reveals Differentially Regulated Protein Phosphorylation Involved in West Nile Virus-Induced Host Inflammatory Response

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Journal of infectious diseases

Application:

IF:

5.226

Species:

Mus musculus

PMID:

24652493

Title:

Etanercept reduces neuroinflammation and lethality in mouse model of Japanese encephalitis

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

8.469

Species:

Homo sapiens

PMID:

28726775

Title:

Oroxylin A activates PKM1/HNF4 alpha to induce hepatoma differentiation and block cancer progression

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

Application:

IF:

5.293

Species:

Homo sapiens

PMID:

28680855

Title:

p21-Activated Kinase 4 Signaling Promotes Japanese Encephalitis Virus-Mediated Inflammation in Astrocytes

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Oncotarget

Application:

IF:

3.765

Species:

Homo sapiens

PMID:

27705941

Title:

MicroRNA-22 negatively regulates poly(I:C)-triggered type I interferon and inflammatory cytokine production via targeting mitochondrial antiviral signaling protein (MAVS)

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Proteomics

Application:

IF:

3.984

Species:

Homo sapiens

PMID:

29251827

Title:

Quantitative Proteomics Identified TTC4 as a TBK1 Interactor and a Positive Regulator of SeV-induced Innate Immunity

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

European review for medical and pharmacological sciences

Application:

IF:

3.507

Species:

Homo sapiens

PMID:

30536301

Title:

Expression of lamin A/C protein in degenerated human intervertebral disc.

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Journal of cellular physiology

Application:

IF:

6.384

Species:

Mus musculus

PMID:

30790292

Title:

Oroxylin A increases the sensitivity of temozolomide on glioma cells by hypoxia-inducible factor 1α/hedgehog pathway under hypoxia.

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Journal of Virology

Application:

IF:

5.103

Species:

Homo sapiens

PMID:

28179530

Title:

Japanese Encephalitis Virus NS5 Inhibits Type I Interferon (IFN) Production by Blocking the Nuclear Translocation of IFN Regulatory Factor 3 and NF-κB

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Nature Communications

Application:

IF:

14.919

Species:

Mus musculus

PMID:

32001717

Title:

The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Cellular Signalling

Application:

IF:

4.315

Species:

Homo sapiens

PMID:

25452104

Title:

miR-206 modulates lipopolysaccharide-mediated inflammatory cytokine production in human astrocytes

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Frontiers in Immunology

Application:

IF:

7.561

Species:

Homo sapiens

PMID:

28717359

Title:

The C-Terminal Effector Domain of Non-Structural Protein 1 of Influenza A Virus Blocks IFN-β Production by Targeting TNF Receptor-Associated Factor 3.

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Am J Cancer Res

Application:

IF:

6.166

Species:

Homo sapiens

PMID:

32509387

Title:

CLIC4 regulates radioresistance of nasopharyngeal carcinoma by iNOS after γ-rays but not carbon ions irradiation

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Genes

Application:

IF:

4.096

Species:

Homo sapiens

PMID:

33158173

Title:

Lymphatic Metastasis of NSCLC Involves Chemotaxis Effects of Lymphatic Endothelial Cells through the CCR7–CCL21 Axis Modulated by TNF-α

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Biomed Pharmacother

Application:

IF:

6.529

Species:

Homo sapiens

PMID:

32428834

Title:

Inhibition of Wnt/β-catenin pathway reverses multi-drug resistance and EMT in Oct4 +/Nanog + NSCLC cells

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Evid Based Complement Alternat Med

Application:

IF:

2.62

Species:

Mus musculus

PMID:

34616480

Title:

Wenshen Shengjing Decoction Improves Early Embryo Development by Maintaining Low H3K27me3 Levels in Sperm and Pronuclear Embryos of Spermatogenesis Impaired Mice

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Front Cell Infect Microbiol

Application:

IF:

5.29

Species:

Sus scrofa

PMID:

32656094

Title:

Porcine Deltacoronavirus Accessory Protein NS7a Antagonizes IFN-β Production by Competing With TRAF3 and IRF3 for Binding to IKKε

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

J Cancer

Application:

IF:

4.207

Species:

Mus musculus

PMID:

34659548

Title:

Bai-He-Gu-Jin-Tang formula suppresses lung cancer via AKT/GSK3β/β-catenin and induces autophagy via the AMPK/mTORC1/ULK1 signaling pathway

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Cell discovery

Application:

IF:

38.07

Species:

Chlorocebus aethiops

PMID:

35790738

Title:

Spatiotemporal regulation of insulin signaling by liquid-liquid phase separation - PMC

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Aging

Application:

IF:

5.95

Species:

Homo sapiens

PMID:

36602530

Title:

lncRNA HCG11 suppresses cell proliferation in hormone receptor-positive breast cancer via SRSF1/β-catenin - PMC

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Homo sapiens

PMID:

36495695

Title:

Inhibition of neutrophil elastase prevents cigarette smoke exposure-induced formation of neutrophil extracellular traps and improves lung function in a mouse …

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Frontiers in cell and developmental biology

Application:

IHC

IF:

6.08

Species:

Mus musculus

PMID:

36819109

Title:

Region-specific heterogeneity in neuronal nuclear morphology in young, aged and in Alzheimers disease mouse brains - PMC

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Autophagy

Application:

IF:

13.39

Species:

Homo sapiens

PMID:

36779599

Title:

Porcine reproductive and respiratory syndrome virus degrades DDX10 via SQSTM1/p62-dependent selective autophagy to antagonize its antiviral activity

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

eLife

Application:

IF:

8.71

Species:

Homo sapiens

PMID:

37498654

Title:

Gαq-PKD/PKCμ signal regulating the nuclear export of HDAC5 to induce the IκB expression and limit the NF-κB-mediated inflammatory response essential for early pregnancy - PMC

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Acta pharmacologica Sinica

Application:

IF:

7.16

Species:

Homo sapiens

PMID:

37402997

Title:

6-Methyl flavone inhibits Nogo-B expression and improves high fructose diet-induced liver injury in mice

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Sci Rep

Application:

WB, IF

IF:

4.996

Species:

Homo sapiens

PMID:

33574425

Title:

The multifunctional role of SPANX-A/D protein subfamily in the promotion of pro-tumoural processes in human melanoma

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

J Biol Chem

Application:

IF:

5.486

Species:

Homo sapiens

PMID:

32913121

Title:

Ascorbic acid enhances low-density lipoprotein receptor expression by suppressing proprotein convertase subtilisin/kexin 9 expression

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Homo sapiens

PMID:

38086852

Title:

Nuclear cGAS restricts L1 retrotransposition by promoting TRIM41-mediated ORF2p ubiquitination and degradation - PMC

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Chinese medicine

Application:

IF:

4.54

Species:

Homo sapiens

PMID:

38360696

Title:

Paclitaxel combined with Compound K inducing pyroptosis of non-small cell lung cancer cells by regulating Treg/Th17 balance - PMC

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Journal of virology

Application:

IF:

5.4

Species:

Homo sapiens,Mus musculus

PMID:

38226815

Title:

Intrahepatic homeobox protein MSX-1 is a novel host restriction factor of hepatitis B virus - PMC

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Journal of orthopaedics and traumatology : official journal of the Italian Society of Orthopaedics and Traumatology

Application:

IHC

IF:

2.8

Species:

Homo sapiens

PMID:

38381214

Title:

The effect of cellular nuclear function alteration on the pathogenesis of shoulder adhesive capsulitis: an immunohistochemical study on lamin A/C expression - PMC

References for Lamin A/C Rabbit pAb (A0249)

Product:

Lamin A/C Rabbit pAb

Journal:

Cancer letters

Application:

IF:

9.7

Species:

Homo sapiens

PMID:

38431034