제품 > 항체 > 다중클론항체(pAb)

MMP3 Rabbit pAb (A1202)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using MMP3 Rabbit pAb (A1202) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from Rat liver, using MMP3 Rabbit pAb (A1202) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - MMP3 Rabbit pAb (A1202)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using MMP3 Rabbit pAb (A1202) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - MMP3 Rabbit pAb (A1202)

Immunofluorescence analysis of C6 cells using MMP3 Rabbit pAb (A1202) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using MMP3 Rabbit pAb (A1202) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	MMP3 Rabbit pAb
Catalog No.	A1202
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 385-477 of human MMP3 (NP_002413.1).
Sequence	VRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC
Gene ID
Swiss Prot
Synonyms	SL-1; STMY; STR1; CHDS6; MMP-3; STMY1; MMP3
Calculated MW	54kDa
Observed MW	60kDa/47kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:100 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Raji, Mouse heart, Rat liver
Cellular location	Secreted, extracellular matrix, extracellular space.
Customer validation	(MB-231) WB(Homo sapiens, Rattus norvegicus, Mus musculus) IHC(Rattus norvegicus, Homo sapiens) IF(Mus musculus ) IF(Rattus norvegicus)

Documents

Certificate of Compliance

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Research Area

MMP3 Rabbit pAb images

Western blot - MMP3 Rabbit pAb (A1202)

Immunohistochemistry - MMP3 Rabbit pAb (A1202)

Immunofluorescence - MMP3 Rabbit pAb (A1202)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A1202

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Reviews List

Product Protocols

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

Journal of Cellular Biochemistry

Application:

IF:

4.429

Species:

Homo sapiens

PMID:

25959126

Title:

C/EBPβ Mediates TNF-α-Induced Cancer Cell Migration by Inducing MMP Expression Dependent on p38 MAPK

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

Exp Cell Res

Application:

IF:

3.905

Species:

Rattus norvegicus

PMID:

32464126

Title:

Exosomal miR-532-5p from bone marrow mesenchymal stem cells reduce intervertebral disc degeneration by targeting RASSF5

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

J Cell Mol Med

Application:

IF:

5.31

Species:

Homo sapiens

PMID:

33764670

Title:

GDF11 prevents the formation of thoracic aortic dissection in mice: Promotion of contractile transition of aortic SMCs

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

European journal of immunology

Application:

IF:

6.68

Species:

Homo sapiens

PMID:

33864383

Title:

Loss of microRNA‐147 function alleviates synovial inflammation through ZNF148 in rheumatoid and experimental arthritis

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

Tissue & cell

Application:

IF:

2.58

Species:

Homo sapiens

PMID:

35623305

Title:

Tectoridin ameliorates proliferation and inflammation in TNF-α-induced HFLS-RA cells via suppressing the TLR4/NLRP3/NF-κB signaling pathway

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IHC

IF:

6.2

Species:

Rattus norvegicus

PMID:

36835440

Title:

Gsmtx4 Alleviated Osteoarthritis through Piezo1/Calcineurin/NFAT1 Signaling Axis under Excessive Mechanical Strain - PMC

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

Gene

Application:

IF:

3.91

Species:

Mus musculus

PMID:

37865148

Title:

What do osteoporosis and osteoarthritis have in common? An integrated study of overlapping differentially expressed genes in bone mesenchymal stem cells of

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

Cell Death Dis

Application:

IF:

9.685

Species:

Homo sapiens

PMID:

32801343

Title:

Homoharringtonine suppresses tumor proliferation and migration by regulating EphB4-mediated -catenin loss in hepatocellular carcinoma

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

Folia histochemica et cytobiologica

Application:

IHC

IF:

1.49

Species:

Homo sapiens

PMID:

36380683

Title:

Wedelolactone ameliorates synovial inflammation and cardiac complications in a murine model of collagen-induced arthritis by inhibiting NF-B/NLRP3

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

Immunity, inflammation and disease

Application:

IF:

2.49

Species:

Rattus norvegicus

PMID:

38156377

Title:

Mechanism of Rhodiola rosea-Euonymus alatus drug pair against rheumatoid arthritis: Network pharmacology and experimental validation - PMC

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

Int Immunopharmacol

Application:

WB, IF

IF:

5.714

Species:

Rattus norvegicus

PMID:

34130151

Title:

Nicotinamide mononucleotide-elicited NAMPT signaling activation aggravated adjuvant-induced arthritis in rats by affecting peripheral immune cells differentiation

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IHC

IF:

5.19

Species:

Rattus norvegicus

PMID:

36209953

Title:

Wutou decoction attenuates the synovial inflammation of collagen-induced arthritis rats via regulating macrophage M1/M2 type polarization

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

J Inflamm Res

Application:

IF:

4.631

Species:

Rattus norvegicus

PMID:

33623411

Title:

Securidaca inappendiculata-Derived Xanthones Protected Joints from Degradation in Male Rats with Collagen-Induced Arthritis by Regulating PPAR- Signaling

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IHC

IF:

7.41

Species:

Rattus norvegicus

PMID:

37634474

Title:

Barasertib impedes chondrocyte senescence and alleviates osteoarthritis by mitigating the destabilization of heterochromatin induced by AURKB

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

International Immunopharmacology

Application:

IF:

5.6

Species:

Rattus norvegicus

PMID:

36252481

Title:

Indole-3-aldehyde alleviates chondrocytes inflammation through the AhR-NF-B signalling pathway

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

Antioxidants & redox signaling

Application:

IF:

6.6

Species:

Rattus norvegicus

PMID:

38504579

Title:

Glutamine mitigates oxidative stress-induced matrix degradation, ferroptosis, and pyroptosis in nucleus pulposus cells via deubiquitinating and stabilizing Nrf2

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Rattus norvegicus

PMID:

39143545

Title:

A structured biomimetic nanoparticle as inflammatory factor sponge and autophagy-regulatory agent against intervertebral disc degeneration and discogenic pain - PMC

References for MMP3 Rabbit pAb (A1202)

Product:

MMP3 Rabbit pAb

Journal:

Experimental neurology

Application:

IF:

5.3

Species:

Mus musculus

PMID:

32991932