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MMP9 Rabbit pAb (A0289)

Reviews (4)Publications (127) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:MMP9 Rabbit pAb
ABclonal:Western blot - MMP9 Rabbit pAb (A0289)

Western blot analysis of lysates from K-562 cells, using MMP9 Rabbit pAb (A0289) at 1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - MMP9 Rabbit pAb (A0289)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using MMP9 Rabbit pAb (A0289) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - MMP9 Rabbit pAb (A0289)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using MMP9 Rabbit pAb (A0289) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - MMP9 Rabbit pAb (A0289)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using MMP9 Rabbit pAb (A0289) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - MMP9 Rabbit pAb (A0289)

Immunofluorescence analysis of L929 cells using MMP9 Rabbit pAb (A0289) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for MMP9 Rabbit pAb(A0289)
Review
ABclonal: review for MMP9 Rabbit pAb(A0289)
Review
ABclonal: review for MMP9 Rabbit pAb(A0289)
Review
ABclonal: review for MMP9 Rabbit pAb(A0289)

Overview

Product nameMMP9 Rabbit pAb
Catalog No.A0289
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 538-707 of human MMP9 (NP_004985.2).
SequenceKYWRFSEGRGSRPQGPFLIADKWPALPRKLDSVFEERLSKKLFFFSGRQVWVYTGASVLGPRRLDKLGLGADVAQVTGALRSGRGKMLLFSGRRLWRFDVKAQMVDPRSASEVDRMFPGVPLDTHDVFQYREKAYFCQDRFYWRVSSRSELNQVDQVGYVTYDILQCPED
Gene ID
Swiss Prot
SynonymsGELB; CLG4B; MMP-9; MANDP2; MMP9
Calculated MW78kDa
Observed MW96kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesK-562
Cellular locationSecreted, extracellular matrix, extracellular space.
Customer validation

(Eoma cell, Human glioma tissue, MDA-MB-231)

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Sus scrofa, Echinococcus multilocularis, Gallus gallus, Arabidopsis thaliana, Mus musculus, Other)

IHC(Mus musculus, Rattus norvegicus)

IHC(Homo sapiens, Gallus gallus, Mus musculus)

IHC(Mus musculus)

IF(Rattus norvegicus)

ELISA(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - MMP9 Rabbit pAb (A0289)}

Western blot - MMP9 Rabbit pAb (A0289)

Western blot analysis of lysates from K-562 cells, using MMP9 Rabbit pAb (A0289) at 1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - MMP9 Rabbit pAb (A0289)}

Immunohistochemistry - MMP9 Rabbit pAb (A0289)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using MMP9 Rabbit pAb (A0289) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - MMP9 Rabbit pAb (A0289)}

Immunohistochemistry - MMP9 Rabbit pAb (A0289)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using MMP9 Rabbit pAb (A0289) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - MMP9 Rabbit pAb (A0289)}

Immunohistochemistry - MMP9 Rabbit pAb (A0289)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using MMP9 Rabbit pAb (A0289) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - MMP9 Rabbit pAb (A0289)}

Immunofluorescence - MMP9 Rabbit pAb (A0289)

Immunofluorescence analysis of L929 cells using MMP9 Rabbit pAb (A0289) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (4)

ELISA 키트 (1)

재조합 단백질 (2)

제품 (2)

Secondary Antibodies (26)