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MSRB2 Rabbit pAb (A8364)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MSRB2 Rabbit pAb (A8364)

Western blot analysis of various lysates using MSRB2 Rabbit pAb (A8364) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - MSRB2 Rabbit pAb (A8364)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using MSRB2 Rabbit pAb (A8364) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameMSRB2 Rabbit pAb
Catalog No.A8364
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable actin binding activity; peptide-methionine (R)-S-oxide reductase activity; and zinc ion binding activity. Predicted to be involved in actin filament polymerization and protein repair. Predicted to be located in mitochondrion. Predicted to be active in cytoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 10-182 of human MSRB2 (NP_036360.3).
SequenceGLTLGTAPRRAVRGQAGGGGPGTGPGLGEAGSLATCELPLAKSEWQKKLTPEQFYVTREKGTEPPFSGIYLNNKEAGMYHCVCCDSPLFSSEKKYCSGTGWPSFSEAHGTSGSDESHTGILRRLDTSLGSARTEVVCKQCEAHLGHVFPDGPGPNGQRFCINSVALKFKPRKH
Gene ID
Swiss Prot
SynonymsCBS1; MSRB; PILB; CBS-1; CGI-131; MSRB2
Calculated MW20kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse heart, Mouse kidney, Mouse brain, Rat liver
Cellular locationMitochondrion
Customer validation

WB(Mus musculus)

IHC(Mus musculus)

Documents

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ABclonal:Western blot - MSRB2 Rabbit pAb (A8364)}

Western blot - MSRB2 Rabbit pAb (A8364)

Western blot analysis of various lysates using MSRB2 Rabbit pAb (A8364) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - MSRB2 Rabbit pAb (A8364)}

Immunohistochemistry - MSRB2 Rabbit pAb (A8364)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using MSRB2 Rabbit pAb (A8364) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A8364? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)