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MVP Rabbit pAb (A1980)

Review (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - MVP Rabbit pAb (A1980)

Western blot analysis of lysates from NIH/3T3 cells, using MVP Rabbit pAb (A1980) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - MVP Rabbit pAb (A1980)

Immunohistochemistry analysis of MVP in paraffin-embedded Human colon tissue using MVP Rabbit pAb (A1980) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Review
ABclonal: review for MVP Rabbit pAb(A1980)

Overview

Product nameMVP Rabbit pAb
Catalog No.A1980
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human MVP (NP_005106.2).
SequenceMATEEFIIRIPPYHYIHVLDQNSNVSRVEVGPKTYIRQDNERVLFAPMRMVTVPPRHYCTVANPVSRDAQGLVLFDVTGQVRLRHADLEIRLAQDPFPLYPGEVLEKDITPLQVVLPNTALHLKALLDFEDKDGDKVVAGDEWLFEGPGTYIPRKEVEVVEIIQATIIRQNQALRLRARKECWDRDGKERVTGEEWLVTTVGAYLPAVFEEVLDLVDAVILTEKTALHLRARRNFRDFRGVSRRTGEEWLVTVQDTEAHV
Gene ID
Swiss Prot
SynonymsLRP; VAULT1; MVP
Calculated MW99kDa
Observed MW110kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesNIH/3T3
Cellular locationCytoplasm, Nucleus, nuclear pore complex
Customer validation

(HepG2)

Documents

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    ABclonal:Western blot - MVP Rabbit pAb (A1980)}

    Western blot - MVP Rabbit pAb (A1980)

    Western blot analysis of lysates from NIH/3T3 cells, using MVP Rabbit pAb (A1980) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - MVP Rabbit pAb (A1980)}

    Immunohistochemistry - MVP Rabbit pAb (A1980)

    Immunohistochemistry analysis of MVP in paraffin-embedded Human colon tissue using MVP Rabbit pAb (A1980) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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