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MYH7/β-MHC Rabbit PolymAb® (A24664PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MYH7/β-MHC Rabbit PolymAb® (A24664PM)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with MYH7 using MYH7/β-MHC Rabbit PolymAb® (A24664PM) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 20s.

ABclonal:Immunofluorescence - MYH7/β-MHC Rabbit PolymAb® (A24664PM)

Confocal imaging of paraffin-embedded Rat heart tissue using MYH7/β-MHC Rabbit PolymAb® (A24664PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - MYH7/β-MHC Rabbit PolymAb® (A24664PM)

Confocal imaging of paraffin-embedded Mouse heart tissue using MYH7/β-MHC Rabbit PolymAb® (A24664PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - MYH7/β-MHC Rabbit PolymAb® (A24664PM)

Confocal imaging of paraffin-embedded Human heart tissue using MYH7/β-MHC Rabbit PolymAb® (A24664PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameMYH7/β-MHC Rabbit PolymAb®
Catalog No.A24664PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing distal myopathy.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1240-1340 of human MYH7/β-MHC (NP_000248.2).
SequenceKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDC
Gene ID
Swiss Prot
SynonymsCMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB; CMYP7A; CMYP7B
Calculated MW223kDa
Observed MW250kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:3000 - 1:12000
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293F transfected with MYH7
Cellular locationCytoplasm, myofibril, sarcomere, Z disc.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - MYH7/β-MHC Rabbit PolymAb® (A24664PM)}

Western blot - MYH7/β-MHC Rabbit PolymAb® (A24664PM)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with MYH7 using MYH7/β-MHC Rabbit PolymAb® (A24664PM) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 20s.
ABclonal:Immunofluorescence - MYH7/β-MHC Rabbit PolymAb® (A24664PM)}

Immunofluorescence - MYH7/β-MHC Rabbit PolymAb® (A24664PM)

Confocal imaging of paraffin-embedded Rat heart tissue using MYH7/β-MHC Rabbit PolymAb® (A24664PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Immunofluorescence - MYH7/β-MHC Rabbit PolymAb® (A24664PM)}

Immunofluorescence - MYH7/β-MHC Rabbit PolymAb® (A24664PM)

Confocal imaging of paraffin-embedded Mouse heart tissue using MYH7/β-MHC Rabbit PolymAb® (A24664PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Immunofluorescence - MYH7/β-MHC Rabbit PolymAb® (A24664PM)}

Immunofluorescence - MYH7/β-MHC Rabbit PolymAb® (A24664PM)

Confocal imaging of paraffin-embedded Human heart tissue using MYH7/β-MHC Rabbit PolymAb® (A24664PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

* For research use only. Not for therapeutic or diagnostic purposes.

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