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Mitofusin-1/MFN1 Rabbit mAb (A21293)

Publications (10) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Mitofusin-1/MFN1 Rabbit mAb (A21293)

Western blot analysis of various lysates using Mitofusin-1/MFN1 Rabbit mAb (A21293) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Mitofusin-1/MFN1 Rabbit mAb (A21293)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Mitofusin-1/MFN1 Rabbit mAb (A21293) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Mitofusin-1/MFN1 Rabbit mAb (A21293)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Mitofusin-1/MFN1 Rabbit mAb (A21293) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameMitofusin-1/MFN1 Rabbit mAb
Catalog No.A21293
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC54223
The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 642-741 of human Mitofusin-1/MFN1 (NP_284941.2).
SequenceFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEEEIARLPKEIDQLEKIQNNSKLLRNKAVQLENELENFTKQFLPSSNEES
Gene ID
Swiss Prot
Synonymshfzo1; hfzo2; Mitofusin-1/MFN1
Calculated MW84kDa
Observed MW84kDa/
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse brain, T-47D, HeLa, A-431
Cellular locationCytoplasm, Mitochondrion outer membrane, Multi-pass membrane protein.
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Fathead minnow, Gallus gallus, Ctenopharyngodon idellus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Mitofusin-1/MFN1 Rabbit mAb (A21293)}

Western blot - Mitofusin-1/MFN1 Rabbit mAb (A21293)

Western blot analysis of various lysates using Mitofusin-1/MFN1 Rabbit mAb (A21293) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Mitofusin-1/MFN1 Rabbit mAb (A21293)}

Immunohistochemistry - Mitofusin-1/MFN1 Rabbit mAb (A21293)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Mitofusin-1/MFN1 Rabbit mAb (A21293) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Mitofusin-1/MFN1 Rabbit mAb (A21293)}

Immunohistochemistry - Mitofusin-1/MFN1 Rabbit mAb (A21293)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Mitofusin-1/MFN1 Rabbit mAb (A21293) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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