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Mouse anti GFP-Tag mAb (AE012)

Publications (205) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Mouse anti GFP-Tag mAb
ABclonal:Western blot - Mouse anti GFP-Tag mAb (AE012)

Western blot analysis of insect expressed GFP protein using Mouse anti GFP-Tag mAb (AE012) at different dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - Mouse anti GFP-Tag mAb (AE012)

Immunoprecipitation of SERPINB1-Flag-GFP from 300 µg extracts of 293T cells transfected with a SERPINB1 expression vector containing a single N-terminal Flag-GFP-Tag was performed using 3 µg of Mouse anti GFP-Tag mAb (AE012). Mouse Control IgG (AC011) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer.The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Rabbit anti DDDDK-Tag mAb (AE063) at a dilution of 1:5000.

ABclonal:Immunoprecipitation - Mouse anti GFP-Tag mAb (AE012)

Immunoprecipitation of SERPINB1-Flag-GFP from 300 µg extracts of 293T cells transfected with a SERPINB1 expression vector containing a single C-terminal Flag-GFP-Tag was performed using 3 µg of Mouse anti GFP-Tag mAb (AE012). Mouse Control IgG (AC011) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer.The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Rabbit anti DDDDK-Tag mAb (AE063) at a dilution of 1:5000.

Overview

Product nameMouse anti GFP-Tag mAb
Catalog No.AE012
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.AMC0483R
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest. GFP can be introduced into animals or other species through transgenic techniques, and maintained in their genome and that of their offspring. To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 to the N-terminus of GFP protein.
SequenceMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFF
Gene ID
Swiss Prot
SynonymsGFP; GFP tag; GFP-tag
Calculated MW27kDa
Observed MW27kDa/68kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:40000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesinsect expression of GFP, 293T
Cellular location
Customer validation

IF(Homo sapiens, Mus musculus, Arabidopsis thaliana, Nicotiana tabacum, Mus musculus, Rattus norvegicus)

WB(Homo sapiens, Saccharomyces cerevisiae, Arabidopsis thaliana, Nicotiana tabacum L, Rosa rugosa Thunb, Yeast, Nicotiana tabacum L., Mus musculus, Other, N. benthamiana, Helicoverpa armigera, Cotton bollworms, Populus, Solanum tuberosum, Oryza sativa, Ictalurus punctatus, Anatinae, N. benthamiana plants, Hordeum vulgare, N. tabacum , Danio rerio, Carassius auratus gibelio, Caenorhabditis elegans, Solanum lycopersicum L, Cynoglossus robustus, tobacco, M. oryzae, Triticum aestivum, Nicotiana benthamiana, Chlorocebus aethiops, Tadarida brasiliensis, Mus musculus, Chlorocebus aethiops, Rattus norvegicus, Stemphylium eturmiunum, Glycine max, Ctenopharyngodon idellus, Agrobacterium, Sus scrofa, Zea mays, Oryctolagus cuniculus, Escherichia coli)

IP(Mus musculus, Triticum aestivum, Nicotiana tabacum, N. benthamiana, Arabidopsis thaliana, Homo sapiens, Ictalurus punctatus, Zea mays)

Co-IP(Homo sapiens, Other, Cucumis sativus , Oryza sativa, Helicoverpa armigera, Glycine max, Malus domestica, Arabidopsis thaliana)

IHC(Homo sapiens, Rattus norvegicus)

Co-IP(Homo sapiens, Stemphylium eturmiunum, Mus musculus, Oryza sativa, Agrobacterium, Other)

IP(Homo sapiens, Other, Anatinae, Arabidopsis thaliana, Chlorocebus aethiops)

IF(Homo sapiens, Mus musculus, Rattus norvegicus, Chlorocebus aethiops)

ChIP(Other, Arabidopsis thaliana, Homo sapiens)

Co-IP(Other)

WB(Other, Homo sapiens)

CUT&Tag(Oryza sativa)

Other(Homo sapiens, Mus musculus, Triticum aestivum)

IHC(Rattus norvegicus, Mus musculus)

Pull-down(Arabidopsis thaliana)

FC(Homo sapiens)

RT-qPCR(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - Mouse anti GFP-Tag mAb (AE012)}

    Western blot - Mouse anti GFP-Tag mAb (AE012)

    Western blot analysis of insect expressed GFP protein using Mouse anti GFP-Tag mAb (AE012) at different dilution.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunoprecipitation - Mouse anti GFP-Tag mAb (AE012)}

    Immunoprecipitation - Mouse anti GFP-Tag mAb (AE012)

    Immunoprecipitation of SERPINB1-Flag-GFP from 300 µg extracts of 293T cells transfected with a SERPINB1 expression vector containing a single N-terminal Flag-GFP-Tag was performed using 3 µg of Mouse anti GFP-Tag mAb (AE012). Mouse Control IgG (AC011) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer.The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Rabbit anti DDDDK-Tag mAb (AE063) at a dilution of 1:5000.
    ABclonal:Immunoprecipitation - Mouse anti GFP-Tag mAb (AE012)}

    Immunoprecipitation - Mouse anti GFP-Tag mAb (AE012)

    Immunoprecipitation of SERPINB1-Flag-GFP from 300 µg extracts of 293T cells transfected with a SERPINB1 expression vector containing a single C-terminal Flag-GFP-Tag was performed using 3 µg of Mouse anti GFP-Tag mAb (AE012). Mouse Control IgG (AC011) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer.The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Rabbit anti DDDDK-Tag mAb (AE063) at a dilution of 1:5000.

    * For research use only. Not for therapeutic or diagnostic purposes.

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