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Mouse anti HA-Tag mAb (AE008)

Publications (189) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Mouse anti HA-Tag mAb
ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008)

Western blot analysis of over-expressed HA-tagged protein in 293T cell using Mouse anti HA-Tag mAb (AE008) at different dilution. Each lane was loaded with 2 ug cell lysate.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008)

Western blot analysis of lysates from 293T cells, using Mouse anti HA-Tag mAb (AE008) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008)

Western blot analysis of lysates from 293T cells, using Mouse anti HA-Tag mAb (AE008) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008)

Western blot analysis of lysates from 293T cells, using Mouse anti HA-Tag mAb (AE008) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008)

Western blot analysis of lysates from 293T cells, using Mouse anti HA-Tag mAb (AE008) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - Mouse anti HA-Tag mAb (AE008)

Immunofluorescence analysis of 293T cells transfected with HA-Tag fusion protein and untreated 293T cells use Mouse anti HA-Tag mAb (AE008) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Mouse anti HA-Tag mAb (AE008)

Immunoprecipitation of over-expressed HA-tagged protein in 293T cells using HA-tag antibody (AE008). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

Overview

Product nameMouse anti HA-Tag mAb
Catalog No.AE008
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.AMC0503
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to HA tag.
SequenceYPYDVPDYA
Gene ID
Swiss Prot
SynonymsHA; HA tag; HA-tag
Calculated MW
Observed MWRefer to Figures
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T-HA(N-terminal), 293T-NFAT(HA-C-terminal)
Cellular location
Customer validation

WB(Homo sapiens, Mus musculus, Mycobacterium tuberculosis, Serpentiformes, Nicotiana tabacum L, Drosophila melanogaster, Sus scrofa, Solanum tuberosum L., Other, Arabidopsis thaliana, Penicillium oxalicum, M. oryzae, Saccharomyces cerevisiae, P. oxalicum, Mus musculus, B. subtilis , Rattus norvegicus, Zea mays, Gallus gallus, Sus scrofa, Danio rerio)

IF(Homo sapiens,Serpentiformes, Cyprinus gibelio, Mus musculus, Drosophila melanogaster)

IP(Mus musculus, Other, Homo sapiens, Vitis vinifera, Sus scrofa, African trypanosomes, Arabidopsis thaliana, Nicotiana tabacum, Zea mays, Cyprinus carpio)

ELISA(Ctenopharyngodon idellus, Mus musculus, Other)

IHC(Mus musculus, Homo sapiens, Mus musculus)

Co-IP(Homo sapiens, Sac fungi , Oryza sativa, Mus musculus, Cucumis sativus, Other, Arabidopsis thaliana, Drosophila melanogaster)

IP(Homo sapiens, Mus musculus, Sus scrofa, Other)

Co-IP(Arabidopsis thaliana, Homo sapiens, Mus musculus, Gallus gallus)

IF(Homo sapiens, Sus scrofa)

RIP(Homo sapiens)

WB(Homo sapiens, Mus musculus, Cyprinus carpio, Drosophila melanogaster)

CUT&Tag(Other)

qPCR(Other)

ChIP(Homo sapiens, Mus musculus)

Other(Oryza sativa, Mus musculus)

IF(Homo sapiens)

IP(Homo sapiens)

Pull-down(Mus musculus)

IHC(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008)}

    Western blot - Mouse anti HA-Tag mAb (AE008)

    Western blot analysis of over-expressed HA-tagged protein in 293T cell using Mouse anti HA-Tag mAb (AE008) at different dilution. Each lane was loaded with 2 ug cell lysate.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008)}

    Western blot - Mouse anti HA-Tag mAb (AE008)

    Western blot analysis of lysates from 293T cells, using Mouse anti HA-Tag mAb (AE008) at 1:5000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008)}

    Western blot - Mouse anti HA-Tag mAb (AE008)

    Western blot analysis of lysates from 293T cells, using Mouse anti HA-Tag mAb (AE008) at 1:10000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008)}

    Western blot - Mouse anti HA-Tag mAb (AE008)

    Western blot analysis of lysates from 293T cells, using Mouse anti HA-Tag mAb (AE008) at 1:5000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008)}

    Western blot - Mouse anti HA-Tag mAb (AE008)

    Western blot analysis of lysates from 293T cells, using Mouse anti HA-Tag mAb (AE008) at 1:10000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunofluorescence - Mouse anti HA-Tag mAb (AE008)}

    Immunofluorescence - Mouse anti HA-Tag mAb (AE008)

    Immunofluorescence analysis of 293T cells transfected with HA-Tag fusion protein and untreated 293T cells use Mouse anti HA-Tag mAb (AE008) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - Mouse anti HA-Tag mAb (AE008)}

    Immunoprecipitation - Mouse anti HA-Tag mAb (AE008)

    Immunoprecipitation of over-expressed HA-tagged protein in 293T cells using HA-tag antibody (AE008). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE008? Please let us know so that we can cite the reference in this datasheet.