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Mouse anti His-Tag mAb (AE003)

Publications (197) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Mouse anti His-Tag mAb
ABclonal:Western blot - Mouse anti His-Tag mAb (AE003)

Western blot analysis of various lysates, using His-Tag mAb (AE003) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - Mouse anti His-Tag mAb (AE003)

Confocal imaging of 293F cells transfected with His-Tag using Mouse anti His-Tag mAb (AE003, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - Mouse anti His-Tag mAb (AE003)

Confocal imaging of 293F cells transfected with His-Tag using Mouse anti His-Tag mAb (AE003, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameMouse anti His-Tag mAb
Catalog No.AE003
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1
CloneNo.AMC0149
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
ImmunogenA synthetic peptide corresponding to His tag.
SequenceHHHHHH
Gene ID
Swiss Prot
SynonymsHis; His tag; His-tag
Calculated MW
Observed MW27kDa, 35kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:20000
  • IF/ICC 1:50-1:200
  • IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T-DLL1-His，293T-CYP1A2-His
Cellular location
Customer validation

WB(Homo sapiens, Crassostrea gigas, Oryctolagus cuniculus, Oryza sativa, Brachyura, Zooxanthella, Mus musculus, Danio rerio, Arabidopsis thaliana, Chlamys Farreri, Sus scrofa, Plasmodium, Other, Helicoverpa armigera, Cotton bollworms, Aspergillus nidulans, Ictalurus punctatus, Escherichia coli, Portunus trituberculatus , R. anatipestifer, Mancinella armigera, Saccharomyces cerevisiae, Cyprinus carpio, Solanum tuberosum , Lactococcus, Malus pumila, Staphylococcus aureus, Mus musculus, Sus scrofa, Triticum aestivum, Canis familiaris, Glycine max, Drosophila melanogaster, Plutella xylostella, Nicotiana benthamiana, Bos taurus, Danio rerio, Ctenopharyngodon idella, Caenorhabditis elegans, Takifugu rubripes, Macaca mulatta, Chlorocebus aethiops)

ELISA(Mus musculus,Rattus norvegicus,Oyster, Cynoglossus semilaevis, Chiloscyllium plagiosum)

IF(Rattus norvegicus, Saccharomyces cerevisiae, Homo sapiens, Mus musculus, Danio rerio)

IP(Cyprinus carpio, Homo sapiens, Other, Arabidopsis thaliana, Saccharomyces cerevisiae)

Co-IP(Danio rerio)

IF(Danio rerio, Homo sapiens, Mus musculus, Ctenopharyngodon idella, Cyprinus carpio)

IHC(Homo sapiens, Mus musculus)

IP(Arabidopsis thaliana, Homo sapiens, Mus musculus, Ictalurus punctatus, Other, Escherichia coli)

Co-IP(Homo sapiens, Helicoverpa armigera)

Co-IP(Other, Mus musculus, Arabidopsis thaliana, Homo sapiens)

WB(Saccharomyces cerevisiae, Other)

Other(Other, Oryza sativa, OystersC. gigas, Danio rerio, Mus musculus)

Pull-down(Arabidopsis thaliana, Oryza sativa, Mus musculus, Triticum aestivum)

WB (Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Mouse anti His-Tag mAb (AE003)}

    Western blot - Mouse anti His-Tag mAb (AE003)

    Western blot analysis of various lysates, using His-Tag mAb (AE003) at 1:5000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - Mouse anti His-Tag mAb (AE003)}

    Immunofluorescence - Mouse anti His-Tag mAb (AE003)

    Confocal imaging of 293F cells transfected with His-Tag using Mouse anti His-Tag mAb (AE003, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - Mouse anti His-Tag mAb (AE003)}

    Immunofluorescence - Mouse anti His-Tag mAb (AE003)

    Confocal imaging of 293F cells transfected with His-Tag using Mouse anti His-Tag mAb (AE003, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE003? Please let us know so that we can cite the reference in this datasheet.