제품 > 항체 > Tag & 로딩 컨트롤 항체

Mouse anti MBP-Tag mAb (AE016)

Publications (38) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Mouse anti MBP-Tag mAb (AE016)

Western blot analysis of recombinant MBP protein using Mouse anti MBP-Tag mAb (AE016) at dilution of 1:5000.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 10 ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameMouse anti MBP-Tag mAb
Catalog No.AE016
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.AMC0505
Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has an approximate molecular mass of 42.5 kilodaltons.
ImmunogenRecombinant protein of MBP-Tag
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsMBP; MBP tag; MBP-tag
Calculated MW42.5 kDa
Observed MW42 kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRecombinant MBP protein
Cellular location
Customer validation

WB(Crassostrea gigas, Mus musculus, Solanum tuberosum , Lycopersicon esculentum, Arabidopsis thaliana, N. benthamiana, T. aestivum, A. alternata, Cronobacter sakazakii, Malus domestica, Oryza sativa, Homo sapiens, Glycine max, Saccharomyces cerevisiae, Triticum aestivum, Other, Chlorocebus aethiops)

IF(Mus musculus)

IHC(Homo sapiens)

Pull-down(Populus tomentosa, aeromonas hydrophila, Mus musculus, Arabidopsis thaliana, Oryza sativa, Triticum aestivum)

Other(Other, Arabidopsis thaliana)

ChIP(Glycine max)

Other(Triticum aestivum)

Co-IP(Saccharomyces cerevisiae)

IP(Homo sapiens, Chlorocebus aethiops)

    ABclonal:Western blot - Mouse anti MBP-Tag mAb (AE016)}

    Western blot - Mouse anti MBP-Tag mAb (AE016)

    Western blot analysis of recombinant MBP protein using Mouse anti MBP-Tag mAb (AE016) at dilution of 1:5000.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 10 ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE016? Please let us know so that we can cite the reference in this datasheet.