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Myc-Tag Rabbit mAb (AE070)

Publications (57) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Myc-Tag Rabbit mAb (AE070)

Western blot analysis of extracts of normal 293T cells, 293T transfected with NLK Protein, using Myc-Tag Rabbit mAb (AE070) at 1:120000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Myc-Tag Rabbit mAb (AE070)

Western blot analysis of extracts of normal 293T cells, 293T transfected with NLK Protein and 293T transfected with PTEN Protein, using Myc-Tag Rabbit mAb (AE070) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Myc-Tag Rabbit mAb (AE070)

Immunofluorescence analysis of HeLa cells (Left: HeLa cells overexpressing recombinant protein with Myc-Tag；Right: Negative control without Myc-Tag) use Rabbit anti Myc-Tag mAb(AE070) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Myc-Tag Rabbit mAb (AE070)

Immunoprecipitation analysis of 300ug extract cell lysate from 293T cells transfected with NLK expression vector containing a Myc-Tag with 3 μg Myc-Tag Rabbit mAb antibody（AE070).Western blot was performed from the immunoprecipitate using Myc-Tag Rabbit mAb antibody（AE070) at 1:5000 dilution.

ABclonal:Immunoprecipitation - Myc-Tag Rabbit mAb (AE070)

Immunoprecipitation analysis of 200ug extracts of 293T cells transfected with NLK expression vector containing a Myc-Tag with 3 μg Myc-Tag Rabbit mAb (AE070 1:100).Western blot was performed from the immunoprecipitate using Myc-Tag Rabbit mAb (AE070) at 1:1000 dilution.

Overview

Product nameMyc-Tag Rabbit mAb
Catalog No.AE070
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5004-12
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to Myc tag.
SequenceEQKLISEEDL
Gene ID
Swiss Prot
SynonymsMyc; Myc tag; Myc-tag; Myc-Tag
Calculated MW
Observed MW58kDa(NLK)/72kDa(YAP1)/55kDa(PTEN)
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:120000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T transfected with Myc-Tag
Cellular location
Customer validation

WB(Other, Homo sapiens, Cyprinus Carpio, Pelteobagrus fulvidraco, Danio rerio, Mus musculus, Gallus gallus, Nicotiana tabacum, Sus scrofa)

IF(Homo sapiens, Mus musculus, Mus musculus, Other)

IP(Homo sapiens, Mus musculus, Other)

IP(Homo sapiens)

IF(Homo sapiens)

Co-IP(Zea mays, Oryza sativa, Arabidopsis thaliana, Homo sapiens, Other)

ChIP(Homo sapiens)

Co-IP(Nicotiana tabacum)

WB(Homo sapiens, Other)

IP(Homo sapiens,Mus musculus)

PCR(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Myc-Tag Rabbit mAb (AE070)}

    Western blot - Myc-Tag Rabbit mAb (AE070)

    Western blot analysis of extracts of normal 293T cells, 293T transfected with NLK Protein, using Myc-Tag Rabbit mAb (AE070) at 1:120000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - Myc-Tag Rabbit mAb (AE070)}

    Western blot - Myc-Tag Rabbit mAb (AE070)

    Western blot analysis of extracts of normal 293T cells, 293T transfected with NLK Protein and 293T transfected with PTEN Protein, using Myc-Tag Rabbit mAb (AE070) at 1:20000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - Myc-Tag Rabbit mAb (AE070)}

    Immunofluorescence - Myc-Tag Rabbit mAb (AE070)

    Immunofluorescence analysis of HeLa cells (Left: HeLa cells overexpressing recombinant protein with Myc-Tag；Right: Negative control without Myc-Tag) use Rabbit anti Myc-Tag mAb(AE070) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - Myc-Tag Rabbit mAb (AE070)}

    Immunoprecipitation - Myc-Tag Rabbit mAb (AE070)

    Immunoprecipitation analysis of 300ug extract cell lysate from 293T cells transfected with NLK expression vector containing a Myc-Tag with 3 μg Myc-Tag Rabbit mAb antibody（AE070).Western blot was performed from the immunoprecipitate using Myc-Tag Rabbit mAb antibody（AE070) at 1:5000 dilution.
    ABclonal:Immunoprecipitation - Myc-Tag Rabbit mAb (AE070)}

    Immunoprecipitation - Myc-Tag Rabbit mAb (AE070)

    Immunoprecipitation analysis of 200ug extracts of 293T cells transfected with NLK expression vector containing a Myc-Tag with 3 μg Myc-Tag Rabbit mAb (AE070 1:100).Western blot was performed from the immunoprecipitate using Myc-Tag Rabbit mAb (AE070) at 1:1000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE070? Please let us know so that we can cite the reference in this datasheet.