제품 > 항체 > 다중클론항체(pAb)

MyoD1 Rabbit pAb (A0671)

Publications (24) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - MyoD1 Rabbit pAb (A0671)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with MyoD1 using MyoD1 Rabbit pAb (A0671) at 1:1000 dilution. 
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameMyoD1 Rabbit pAb
Catalog No.A0671
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 226-300 of human MyoD1 (NP_002469.2).
SequenceYEGAYYNEAPSEPRPGKSAAVSSLDCLSSIVERISTESPAAPALLLADVPSESPPRRQEAAAPSEGESSGDPTQS
Gene ID
Swiss Prot
SynonymsPUM; MYF3; MYOD; CMYP17; bHLHc1; MYODRIF; MyoD1
Calculated MW35kDa
Observed MW45kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular locationNucleus.
Customer validation

IF(Sus scrofa, Bos taurus)

WB(Mus musculus, Sus scrofa, Rattus norvegicus, Homo sapiens, Sus scrofa)

RT-qPCR(Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - MyoD1 Rabbit pAb (A0671)}

Western blot - MyoD1 Rabbit pAb (A0671)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with MyoD1 using MyoD1 Rabbit pAb (A0671) at 1:1000 dilution. 
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0671? Please let us know so that we can cite the reference in this datasheet.