Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with MyoD1 using MyoD1 Rabbit pAb (A0671) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
| Product name | MyoD1 Rabbit pAb |
|---|---|
| Catalog No. | A0671 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 226-300 of human MyoD1 (NP_002469.2). |
|---|---|
| Sequence | YEGAYYNEAPSEPRPGKSAAVSSLDCLSSIVERISTESPAAPALLLADVPSESPPRRQEAAAPSEGESSGDPTQS |
| Gene ID | |
| Swiss Prot | |
| Synonyms | PUM; MYF3; MYOD; CMYP17; bHLHc1; MYODRIF; MyoD1 |
| Calculated MW | 35kDa |
| Observed MW | 45kDa |
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | 293T |
| Cellular location | Nucleus. |
| Customer validation | IF(Sus scrofa, Bos taurus) WB(Mus musculus, Sus scrofa, Rattus norvegicus, Homo sapiens, Sus scrofa) RT-qPCR(Mus musculus) IF(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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