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N6-methyladenosine / m6A Mouse mAb (A19136)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Dot Blot - N6-methyladenosine / m6A Mouse mAb (A19136)

The m6A mouse monoclonal antibody (A19136) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine (100ng for each oligomer).
Oligomer 8 - N6-methyladenosine (ATAACTGG-m6A-CCGAATGG)
Oligomer 9 - unmodified adenosine (ATAACTGGACCGAATGG)
Oligomer 10 - unmodified adenosine (AAAAAAAAAAAAAAAA-biotin)

Overview

Product nameN6-methyladenosine / m6A Mouse mAb
Catalog No.A19136
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1, kappa
CloneNo.AMC0257
Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)
ImmunogenChemical compounds corresponding to N6-methyladenosine / m6A.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsN6-methyladenosine; m6A; N6-methyladenosine / m6A
Calculated MW
Observed MW
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • DB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application
Positive samples
Cellular location
Customer validation

WB(Mus musculus)

    ABclonal:Dot Blot - N6-methyladenosine / m6A Mouse mAb (A19136)}

    Dot Blot - N6-methyladenosine / m6A Mouse mAb (A19136)

    The m6A mouse monoclonal antibody (A19136) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine (100ng for each oligomer).
    Oligomer 8 - N6-methyladenosine (ATAACTGG-m6A-CCGAATGG)
    Oligomer 9 - unmodified adenosine (ATAACTGGACCGAATGG)
    Oligomer 10 - unmodified adenosine (AAAAAAAAAAAAAAAA-biotin)

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A19136? Please let us know so that we can cite the reference in this datasheet.