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N6-methyladenosine / m6A Rabbit mAb (A22411)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Species independent

The N6-methyladenosine / m6A Rabbit mAb (4µg, A19841) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).
Oligomer 4 - N6-methyladenosine (m6A-UAACUGGACCGAAUGG-Biotin)
Oligomer 5 - N6-methyladenosine (AUAACUGG-m6A-CCGAAUGG-Biotin)
Oligomer 6 - unmodified adenosine (AUAACUGGACCGAAUGG-Biotin)

The N6-methyladenosine / m6A Rabbit mAb (A22411) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.
Oligomer 8 - ATAACTGG-m6A-CCGAATGG
Oligomer 9 - ATAACTGGACCGAATGG
Oligomer 10 - AAAAAAAAAAAAAAAA-biotin.

Dot-blot analysis of all sorts of peptides using N6-methyladenosine / m6A antibody (A22411) at dilution.

ABclonal:Immunofluorescence - N6-methyladenosine / m6A Rabbit mAb (A22411)

U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 °C for the indicated time, immunofluorescence analysis was performed by N6-methyladenosine / m6A Rabbit mAb (A22411), DAPI, 4′, 6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel, peaking at 2 min after irradiation and diminishing over the following 8 min.

Overview

Product name	N6-methyladenosine / m6A Rabbit mAb
Catalog No.	A22411
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC5003-03

Background

Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)

Immunogen information

Immunogen	Chemical compounds corresponding to N6-methyladenosine / m6A.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	N6-methyladenosine; m6A; N6-methyladenosine / m6A
Calculated MW
Observed MW	Refer to figures

Applications

Reactivity	Species independent
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	DB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. meRIP 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Immunofluorescence
Positive samples	N6-methyladenosine / m6A, U-2 OS treated with UV(30s)Brdu(10min) cells
Cellular location
Customer validation	Other(Other)