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NCOA4 Rabbit pAb (A5695)

Publications (26) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NCOA4 Rabbit pAb (A5695)

Western blot analysis of lysates from Rat testis using NCOA4 Rabbit pAb (A5695) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - NCOA4 Rabbit pAb (A5695)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using NCOA4 Rabbit pAb (A5695) at a dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - NCOA4 Rabbit pAb (A5695)

Immunofluorescence analysis of PC-3 cells using NCOA4 Rabbit pAb(A5695) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NCOA4 Rabbit pAb (A5695)

Immunofluorescence analysis of K-562 cells using NCOA4 Rabbit pAb(A5695) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NCOA4 Rabbit pAb (A5695)

Immunofluorescence analysis of 293F cells using NCOA4 Rabbit pAb(A5695) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNCOA4 Rabbit pAb
Catalog No.A5695
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NCOA4 (NP_005428.1).
SequenceMNTFQDQSGSSSNREPLLRCSDARRDLELAIGGVLRAEQQIKDNLREVKAQIHSCISRHLECLRSREVWLYEQVDLIYQLKEETLQQQAQQLYSLLGQFNCLTHQLECTQNKDLANQVSVCLERLGSLTLKPEDSTVLLFEADTITLRQTITTFGSLKTIQIPEHLMAHASSANIGPFLEKRGCISMPEQKSASGIVAVPFSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQTLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSS
Gene ID
Swiss Prot
SynonymsRFG; ELE1; PTC3; ARA70; NCOA4
Calculated MW70kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRat testis
Cellular locationnucleus.
Customer validation

WB(Homo sapiens, Gallus gallus, Mus musculus, Rattus norvegicus, Mus musculus, Rattus norvegicus, Other)

IHC(Mus musculus, Homo sapiens, Mus musculus)

IHC(Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus)

IP(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - NCOA4 Rabbit pAb (A5695)}

Western blot - NCOA4 Rabbit pAb (A5695)

Western blot analysis of lysates from Rat testis using NCOA4 Rabbit pAb (A5695) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Immunohistochemistry - NCOA4 Rabbit pAb (A5695)}

Immunohistochemistry - NCOA4 Rabbit pAb (A5695)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using NCOA4 Rabbit pAb (A5695) at a dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - NCOA4 Rabbit pAb (A5695)}

Immunofluorescence - NCOA4 Rabbit pAb (A5695)

Immunofluorescence analysis of PC-3 cells using NCOA4 Rabbit pAb(A5695) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NCOA4 Rabbit pAb (A5695)}

Immunofluorescence - NCOA4 Rabbit pAb (A5695)

Immunofluorescence analysis of K-562 cells using NCOA4 Rabbit pAb(A5695) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NCOA4 Rabbit pAb (A5695)}

Immunofluorescence - NCOA4 Rabbit pAb (A5695)

Immunofluorescence analysis of 293F cells using NCOA4 Rabbit pAb(A5695) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A5695? Please let us know so that we can cite the reference in this datasheet.