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N-Cadherin Rabbit pAb (A0433)

Review (1)Publications (38) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - N-Cadherin Rabbit pAb (A0433)

Western blot analysis of lysates from Mouse heart, using N-Cadherin Rabbit pAb (A0433) at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - N-Cadherin Rabbit pAb (A0433)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using N-Cadherin Rabbit pAb (A0433) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - N-Cadherin Rabbit pAb (A0433)

Immunohistochemistry analysis of paraffin-embedded Rat heart using N-Cadherin Rabbit pAb (A0433) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Review
ABclonal: review for N-Cadherin Rabbit pAb(A0433)

Overview

Product nameN-Cadherin Rabbit pAb
Catalog No.A0433
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 807-906 of human N-Cadherin (NP_001783.2).
SequenceRRMDERPIHAEPQYPVRSAAPHPGDIGDFINEGLKAADNDPTAPPYDSLLVFDYEGSGSTAGSLSSLNSSSSGGEQDYDYLNDWGPRFKKLADMYGGGDD
Gene ID
Swiss Prot
SynonymsCDHN; NCAD; ACOGS; ADHD8; CD325; ARVD14; CDw325; N-Cadherin
Calculated MW100kDa
Observed MW140kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse heart
Cellular locationCell membrane, Single-pass type I membrane protein.
Customer validation

(U87MG)

WB(Homo sapiens, Mus musculus, Mus musculus, Rattus norvegicus)

IHC(Mus musculus)

IHC(Homo sapiens, Rattus norvegicus)

IF(Homo sapiens)

IF(Homo sapiens)

WB(Mus musculus)

Documents

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ABclonal:Western blot - N-Cadherin Rabbit pAb (A0433)}

Western blot - N-Cadherin Rabbit pAb (A0433)

Western blot analysis of lysates from Mouse heart, using N-Cadherin Rabbit pAb (A0433) at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - N-Cadherin Rabbit pAb (A0433)}

Immunohistochemistry - N-Cadherin Rabbit pAb (A0433)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using N-Cadherin Rabbit pAb (A0433) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - N-Cadherin Rabbit pAb (A0433)}

Immunohistochemistry - N-Cadherin Rabbit pAb (A0433)

Immunohistochemistry analysis of paraffin-embedded Rat heart using N-Cadherin Rabbit pAb (A0433) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)