제품 > 항체 > 단일클론항체(mAb)

NF-kB p65/RelA Mouse mAb (A10609)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using NF-kB p65/RelA Mouse mAb (A10609) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - NF-kB p65/RelA Mouse mAb (A10609)

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review

Overview

Product name	NF-kB p65/RelA Mouse mAb
Catalog No.	A10609
Host species	Mouse
Purification method	Affinity purification
Isotype	IgG1
CloneNo.	AMC0486

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant protein of human NF-kB p65
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	p65; CMCU; NFKB3; AIF3BL3; NF-kB p65/RelA
Calculated MW	60kDa
Observed MW	70kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IF/ICC 1:100 - 1:800 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa, Jurkat, HT-29, SGC-7901, NIH/3T3, Mouse liver
Cellular location	Cytoplasm, Nucleus.
Customer validation	(Mononuclear macrophages, human cells) WB(Homo sapiens, Mus musculus, Edwardsiella tarda, Mauremys sinensis , Cyprinus carpio, Rattus norvegicus, Rattus norvegicus , Mus musculus, Sus scrofa, Other) IF(Mus musculus, Rattus norvegicus, Homo sapiens) IHC(Rattus norvegicus, Mus musculus) WB(Homo sapiens) IHC(Sus scrofa) IF(Mus musculus, Rattus norvegicus, Homo sapiens) Co-IP(Homo sapiens)

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Research Area

NF-kB p65/RelA Mouse mAb images

Western blot - NF-kB p65/RelA Mouse mAb (A10609)

Immunofluorescence - NF-kB p65/RelA Mouse mAb (A10609)

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - NF-kB p65/RelA Mouse mAb (A10609)

Immunofluorescence analysis of C6 cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - NF-kB p65/RelA Mouse mAb (A10609)

Immunofluorescence analysis of U2OS cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A10609

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Reviews List

Product Protocols

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Journal of virology

Application:

IF:

5.103

Species:

Homo sapiens

PMID:

29769346

Title:

Porcine deltacoronavirus accessory protein NS6 antagonizes IFN-β production by interfering with the binding of RIG-I/MDA5 to double-stranded RNA

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Nature communications

Application:

IF:

14.919

Species:

Mus musculus

PMID:

30348973

Title:

Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing.

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Journal of Immunology (Baltimore, Md. : 1950)

Application:

IF:

5.422

Species:

Mus musculus

PMID:

32303555

Title:

CALML6 Controls TAK1 Ubiquitination and Confers Protection against Acute Inflammation

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

International Journal of Molecular Sciences

Application:

IF:

5.923

Species:

Mus musculus,Edwardsiella tarda

PMID:

31731575

Title:

A Comparative Analysis of Edwardsiella tarda-Induced Transcriptome Profiles in RAW264.7 Cells Reveals New Insights into the Strategy of Bacterial Immune Evasion.

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

J Inflamm Res

Application:

IF:

6.922

Species:

Mus musculus

PMID:

32801828

Title:

Therapeutic Effect of CC Chemokine Receptor Type 1 (CCR1) Antagonist BX471 on Allergic Rhinitis

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Adv Sci (Weinh)

Application:

IF:

16.806

Species:

Mus musculus

PMID:

32995120

Title:

RNAi Screening Identifies that TEX10 Promotes the Proliferation of Colorectal Cancer Cells by Increasing NF‐κB Activation

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Aquat Toxicol

Application:

IF:

4.964

Species:

Mauremys sinensis

PMID:

33129562

Title:

Effects of ammonia exposure on antioxidant function, immune response and NF-κB pathway in Chinese Strip-necked Turtle (Mauremys sinensis)

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Comparative Biochemistry And Physiology C-Toxicology & Pharmacology

Application:

IF:

3.228

Species:

Cyprinus carpio

PMID:

33278595

Title:

Saikosaponin a attenuates lead-induced kidney injury through activating Nrf2 signaling pathway

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Integr Cancer Ther

Application:

IHC

IF:

3.279

Species:

Rattus norvegicus

PMID:

33118377

Title:

Ginkgo biloba Extract Attenuates Methotrexate-Induced Testicular Injury in Rats: Cross-talk Between Oxidative Stress, Inflammation, Apoptosis, and miRNA-29a Expression

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Eur J Med Chem

Application:

IF:

6.51

Species:

Rattus norvegicus

PMID:

33784603

Title:

Design, synthesis, and biological evaluation of a novel dual peroxisome proliferator-activated receptor alpha/delta agonist for the treatment of diabetic kidney disease through anti-inflammatory mechanisms

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Life Sci

Application:

IF:

5.03

Species:

Rattus norvegicus

PMID:

33965378

Title:

Asiatic acid attenuates diabetic retinopathy through TLR4/MyD88/NF-κB p65 mediated modulation of microglia polarization

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Int Immunopharmacol

Application:

IHC

IF:

4.93

Species:

Mus musculus

PMID:

34678694

Title:

Bixin protects mice against bronchial asthma though modulating PI3K/Akt pathway

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

J Ethnopharmacol

Application:

IF:

4.36

Species:

Mus musculus

PMID:

34116185

Title:

Anneslea fragrans Wall. ameliorates ulcerative colitis via inhibiting NF-κB and MAPK activation and mediating intestinal barrier integrity

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

ACS Appl Mater Interfaces

Application:

IF:

9.229

Species:

Mus musculus,Rattus norvegicus

PMID:

34618442

Title:

Layered Double Hydroxide Nanoparticles with Osteogenic Effects as miRNA Carriers to Synergistically Promote Osteogenesis of MSCs

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Toxicol Appl Pharmacol

Application:

IF:

4.219

Species:

Mus musculus

PMID:

34755645

Title:

Indoprofen exerts a potent therapeutic effect against sepsis by alleviating high mobility group box 1-mediated inflammatory responses

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Evid Based Complement Alternat Med

Application:

IF:

2.629

Species:

Mus musculus

PMID:

34335857

Title:

Protective Effects of Anwulignan against HCl/Ethanol-Induced Acute Gastric Ulcer in Mice

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Biomed Res Int

Application:

IF:

3.411

Species:

Rattus norvegicus

PMID:

34350293

Title:

Celastrol Attenuates Learning and Memory Deficits in an Alzheimer's Disease Rat Model

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Eur J Pharmacol

Application:

IF:

4.432

Species:

Mus musculus

PMID:

34492285

Title:

Study on the effect of active components of Schisandra chinensis on liver injury and its mechanisms in mice based on network pharmacology

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Immunopharmacology and immunotoxicology

Application:

IF:

3.71

Species:

Mus musculus

PMID:

35980837

Title:

Corynoline ameliorates dextran sulfate sodium-induced colitis in mice by modulating Nrf2/NF-κB pathway

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Molecular human reproduction

Application:

IF:

4.51

Species:

Homo sapiens

PMID:

34427668

Title:

Differential expression of microRNAs in human endometrium after implantation of an intrauterine contraceptive device containing copper

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Biochemical pharmacology

Application:

IHC

IF:

6.1

Species:

Mus musculus

PMID:

35988735

Title:

Schisandrol B protects against cholestatic liver injury by inhibiting pyroptosis through pregnane X receptor

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Medicine

Application:

IF:

1.81

Species:

Homo sapiens

PMID:

36596012

Title:

Effect of Xanthii Fructus alcohol extract on proliferation and apoptosis of HFLS-RA and its mechanism - PMC

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Theriogenology

Application:

IF:

2.92

Species:

Homo sapiens

PMID:

35605398

Title:

Protective mechanism of selenium on mercuric chloride-induced testis injury in chicken via p38 MAPK/ATF2/iNOS signaling pathway

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Immunopharmacology and immunotoxicology

Application:

IF:

3.71

Species:

Mus musculus

PMID:

35506641

Title:

Tectoridin alleviates lipopolysaccharide-induced inflammation via inhibiting TLR4-NF-κB/NLRP3 signaling in vivo and in vitro

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

mBio

Application:

IF:

Species:

Mus musculus

PMID:

36321836

Title:

MIR155HG Plays a Bivalent Role in Regulating Innate Antiviral Immunity by Encoding Long Noncoding RNA-155 and microRNA-155-5p

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Foods (Basel, Switzerland)

Application:

IF:

5.56

Species:

Mus musculus

PMID:

36553716

Title:

Diverse Galactooligosaccharides Differentially Reduce LPS-Induced Inflammation in Macrophages - PMC

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Rattus norvegicus

PMID:

37021049

Title:

Chrysin ameliorates synovitis and fibrosis of osteoarthritic fibroblast-like synoviocytes in rats through PERK/TXNIP/NLRP3 signaling - PMC

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Archives of virology

Application:

IF:

2.68

Species:

Mus musculus

PMID:

37233865

Title:

Japanese encephalitis virus induces apoptosis by activating the RIG-1 signaling pathway

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

The Journal of nutritional biochemistry

Application:

IF:

6.11

Species:

Homo sapiens

PMID:

37178812

Title:

Transcriptomic Evidence of Hypothalamus for Maternal Fructose Exposure Induced Offspring Hypertension through AT1R/TLR4 Pathway

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Diabetes, metabolic syndrome and obesity : targets and therapy

Application:

IF:

3.24

Species:

Homo sapiens,Rattus norvegicus

PMID:

37309505

Title:

Accelerated Wound Healing in Diabetic Rat by miRNA-185-5p and Its Anti-Inflammatory Activity - PMC

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Journal of medicinal chemistry

Application:

IF:

8.03

Species:

Mus musculus

PMID:

37243609

Title:

Discovery of the First Subnanomolar PPARα/δ Dual Agonist for the Treatment of Cholestatic Liver Diseases

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Carbohydrate polymers

Application:

IF:

10.72

Species:

Mus musculus

PMID:

37364951

Title:

Structural analysis and attenuates hyperuricemic nephropathy of dextran from the Imperata cylindrica Beauv. var. major (Nees) CE Hubb.

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

International journal of biological macromolecules

Application:

IF:

8.02

Species:

Homo sapiens, Mus musculus

PMID:

36252620

Title:

CRM197-conjugated multi antigen dominant epitope for effective human cytomegalovirus vaccine development

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

iScience

Application:

IF, WB

IF:

6.1

Species:

Homo sapiens

PMID:

38303710

Title:

Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9 - PMC

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Bioorganic chemistry

Application:

IF:

5.3

Species:

Mus musculus

PMID:

38368656

Title:

Structural modification of natural axinelline A: Achieving reduced colitis side effects through balanced COX inhibition

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Journal of cellular and molecular medicine

Application:

IF, WB

IF:

5.29

Species:

Homo sapiens

PMID:

38214430

Title:

EEBR induces Caspase-1-dependent pyroptosis through the NF-B/NLRP3 signalling cascade in non-small cell lung cancer - PMC

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

The Journal of Pharmacy and Pharmacology

Application:

WB, IHC

IF:

4.81

Species:

Sus scrofa

PMID:

32314371

Title:

Tectorigenin inhibits inflammation and pulmonary fibrosis in allergic asthma model of ovalbumin-sensitized guinea pigs

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Planta Med

Application:

WB, IF

IF:

3.007

Species:

Mus musculus

PMID:

34293804

Title:

Suffrutines A and B Inhibit the Expression of Inflammatory Mediators in LPS-Induced RAW264. 7 Cells by Suppressing the NF-B Signaling Pathway

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Immunopharmacol

Application:

IF:

5.714

Species:

Mus musculus

PMID:

33744777

Title:

Loganin alleviates sepsis-induced acute lung injury by regulating macrophage polarization and inhibiting NLRP3 inflammasome activation

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Bioengineered

Application:

IF:

6.832

Species:

Mus musculus

PMID:

33034242

Title:

Loganin inhibits macrophage M1 polarization and modulates sirt1/NF-B signaling pathway to attenuate ulcerative colitis

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

WB, IF

IF:

6.65

Species:

Rattus norvegicus

PMID:

35490493

Title:

Protective effects of E Se tea extracts against alcoholic fatty liver disease induced by high fat/alcohol diet: In vivo biological evaluation and molecular docking study

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Acta tropica

Application:

IF:

3.22

Species:

Mus musculus

PMID:

35963313

Title:

Toxoplasma gondii rhoptry protein (TgROP18) enhances the expression of pro-inflammatory factor in LPS/IFN--induced murine BV2 microglia cells via NF-B signal

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Journal of cardiovascular translational research

Application:

WB, IF, Co-IP

IF:

3.21

Species:

Homo sapiens

PMID:

35478454

Title:

LncRNA LINC00281/Annexin A2 Regulates Vascular Smooth Muscle Cell Phenotype Switching via the Nuclear Factor-Kappa B Signaling Pathway

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

International journal of biological macromolecules

Application:

IF:

8.2

Species:

Sus scrofa

PMID:

38702002

Title:

Porcine TLR8 signaling and its anti-infection function are disturbed by immune checkpoint receptor TIM-3 via inhibition of P13K-AKT pathway

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

International journal of molecular sciences

Application:

IF:

5.6

Species:

Homo sapiens

PMID:

39000436

Title:

Role of Ciminalum-4-thiazolidinone Hybrids in Molecular NF-B Dependent Pathways - PMC

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

International journal of molecular sciences

Application:

IF:

5.6

Species:

Other

PMID:

38791533

Title:

Comparison of the Antioxidant and Cytoprotective Properties of Extracts from Different Cultivars of Cornus mas L. - PMC

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

iScience

Application:

IF:

5.8

Species:

Mus musculus

PMID:

39184442

Title:

Astroglia proliferate upon the biogenesis of tunneling nanotubes via -synuclein dependent transient nuclear translocation of focal adhesion kinase - PMC

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Food Res Int

Application:

IF:

8.1

Species:

Mus musculus

PMID:

34980415

Title:

Ink melanin from Sepiapharaonis ameliorates colitis in mice via reducing oxidative stress, andprotecting the intestinal mucosal barrier

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Pharmaceutics

Application:

IF:

5.4

Species:

Homo sapiens

PMID:

35631550

Title:

The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death

References for NF-kB p65/RelA Mouse mAb (A10609)

Product:

NF-kB p65/RelA Mouse mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.7

Species:

Mus musculus,Rattus norvegicus

PMID:

38564920