제품 > 항체 > 다중클론항체(pAb)

NF-kB p65/RelA Rabbit pAb (A11204)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from 293T cells, using NF-kB p65/RelA Rabbit pAb (A11204) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A11204)

Immunofluorescence analysis of U2OS cells using NF-kB p65/RelA Rabbit pAb (A11204) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Rabbit pAb (A11204) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using NF-kB p65/RelA Rabbit pAb (A11204) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using NF-kB p65/RelA Rabbit pAb (A11204) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - NF-kB p65/RelA Rabbit pAb (A11204)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg NF-kB p65/RelA antibody (A11204). Western blot was performed from the immunoprecipitate using NF-kB p65/RelA antibody (A11204) at a dilution of 1:1000.

Review

Overview

Product name	NF-kB p65/RelA Rabbit pAb
Catalog No.	A11204
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 450-551 of human NF-kB p65/RelA (NP_068810.3).
Sequence	LGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS
Gene ID
Swiss Prot
Synonyms	p65; CMCU; NFKB3; AIF3BL3; NF-kB p65/RelA
Calculated MW	60kDa
Observed MW	65kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	293T
Cellular location	Cytoplasm, Nucleus
Customer validation	(C57BL6) IF(Homo sapiens, Mus musculus) WB(Homo sapiens, Mus musculus, Sus scrofa, Rattus norvegicus)

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NF-kB p65/RelA Rabbit pAb images

Western blot - NF-kB p65/RelA Rabbit pAb (A11204)

Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A11204)

Immunofluorescence analysis of U2OS cells using NF-kB p65/RelA Rabbit pAb (A11204) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A11204)

Immunoprecipitation - NF-kB p65/RelA Rabbit pAb (A11204)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Experimental Cell Research

Application:

IF:

3.905

Species:

Homo sapiens

PMID:

28733145

Title:

Adipocytes promote nicotine-induced injury of endothelial cells via the NF-κB pathway

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

Application:

IF:

5.293

Species:

Homo sapiens

PMID:

28680855

Title:

p21-Activated Kinase 4 Signaling Promotes Japanese Encephalitis Virus-Mediated Inflammation in Astrocytes

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

International Journal Of Biochemistry & Cell Biology

Application:

IF:

5.085

Species:

Mus musculus

PMID:

28789920

Title:

The ω-carboxyl group of 7-ketocholesteryl-9-carboxynonanoate mediates the binding of oxLDL to CD36 receptor and enhances caveolin-1 expression in macrophages

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Pulm Pharmacol Ther

Application:

IF:

3.41

Species:

Mus musculus

PMID:

33887491

Title:

Gentiopicroside ameliorates ovalbumin-induced airway inflammation in a mouse model of allergic asthma via regulating SIRT1/NF-κB signaling pathway

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Antioxidants (Basel)

Application:

IF:

6.31

Species:

Mus musculus

PMID:

34572964

Title:

Discovery of Novel Pterostilbene Derivatives That Might Treat Sepsis by Attenuating Oxidative Stress and Inflammation through Modulation of MAPKs/NF-κB Signaling Pathways

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Environ Toxicol

Application:

IF:

4.11

Species:

Mus musculus

PMID:

34018660

Title:

Effects of manganese on microglia M1/M2 polarization and SIRT1-mediated transcription of STAT3-dependent genes in mouse

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Food Funct

Application:

IF:

5.396

Species:

Mus musculus

PMID:

34057171

Title:

Preparation, characterization, and in vivo evaluation of anti-inflammatory activities of selenium nanoparticles synthesized by Kluyveromyces lactis GG799

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Environmental toxicology

Application:

IF:

4.1

Species:

Sus scrofa

PMID:

35388968

Title:

Glyphosate-based herbicides induces autophagy in IPEC-J2 cells and the intervention of N-acetylcysteine

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

European journal of medicinal chemistry

Application:

IF:

7.08

Species:

Mus musculus

PMID:

36215862

Title:

Discovery of novel AdipoRon analogues as potent anti-inflammatory agents against nonalcoholic steatohepatitis

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Scientific reports

Application:

IF:

4.99

Species:

Mus musculus

PMID:

36380080

Title:

Lentinan improves intestinal inflammation and gut dysbiosis in antibiotics-induced mice - PMC

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Frontiers in immunology

Application:

IF:

8.78

Species:

Homo sapiens

PMID:

36814917

Title:

Single-cell RNA sequencing reveals the immune microenvironment and signaling networks in cystitis glandularis - PMC

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

European journal of pharmacology

Application:

IF:

5.19

Species:

Rattus norvegicus

PMID:

37179040

Title:

Oroxin B improves metabolic-associated fatty liver disease by alleviating gut microbiota dysbiosis in a high-fat diet-induced rat model

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Infection and drug resistance

Application:

IF:

4.17

Species:

Sus scrofa

PMID:

37404255

Title:

18-Glycyrrhetinic Acid Alleviates P. multocida-Induced Vascular Endothelial Inflammation by PARP1-Mediated NF-B and HMGB1 Signalling Suppression in PIEC Cells - PMC

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Carbohydr Polym .

Application:

IF:

10.723

Species:

Mus musculus

PMID:

33838836

Title:

Value added immunoregulatory polysaccharides of Hericium erinaceus and their effect on the gut microbiota

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Vet Microbiol

Application:

IF:

3.246

Species:

Sus scrofa

PMID:

34411995

Title:

Deletion of the crp gene affects the virulence and the activation of the NF-B and MAPK signaling pathways in PK-15 and iPAM cells derived from G. parasuis serovar 5

References for NF-kB p65/RelA Rabbit pAb (A11204)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Immunity, inflammation and disease

Application:

IF:

2.49

Species:

Homo sapiens

PMID:

37249279