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NF-kB p65/RelA Rabbit pAb (A2547)

Reviews (6)Publications (155) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NF-kB p65/RelA Rabbit pAb (A2547)

Western blot analysis of various lysates using NF-kB p65/RelA Rabbit pAb (A2547) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - NF-kB p65/RelA Rabbit pAb (A2547)

Western blot analysis of various lysates, using NF-kB p65/RelA Rabbit pAb (A2547) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Western blot - NF-kB p65/RelA Rabbit pAb (A2547)

Western blot analysis of lysates from wild type(WT) and NF-kB p65/RelA knockout (KO) HeLa(KO) cells, using NF-kB p65/RelA Rabbit pAb (A2547) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry analysis of paraffin-embedded Human colon using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry analysis of paraffin-embedded Rat testis using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry analysis of paraffin-embedded Human colon using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry analysis of paraffin-embedded Mouse colon using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry analysis of paraffin-embedded Rat heart using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of HepG2 cells using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of HepG2 cells using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A2547)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A2547)

Overview

Product nameNF-kB p65/RelA Rabbit pAb
Catalog No.A2547
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 450-550 of human NF-kB p65/RelA (NP_068810.3).
SequenceLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQIS
Gene ID
Swiss Prot
Synonymsp65; CMCU; NFKB3; AIF3BL3; NF-kB p65/RelA
Calculated MW60kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, MCF7, Mouse lung, Mouse kidney, Rat lung, Rat kidney
Cellular locationCytoplasm, Nucleus.
Customer validation

(RAW264.7, Mouse atherosclerosis arterial tissue, HCC827, U251, Rat H9c2 cells)

WB(Homo sapiens, Macaca mulatta, Rattus norvegicus, Sus scrofa, Schisandra chinensis, Mus musculus, Alpinia katsumadai Hayata, Bos taurus, Gallus gallus, King Charles Spaniel, Mus musculus, Cryptosporidium parvum, Other)

IF(Mus musculus, Rattus norvegicus, Homo sapiens)

IHC(Mus musculus, Rattus norvegicus, Homo sapiens)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Rattus norvegicus, Mus musculus)

WB(Rattus norvegicus, Mus musculus, Homo sapiens)

IHC(Rattus norvegicus, Mus musculus)

ChIP(Homo sapiens)

Other(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - NF-kB p65/RelA Rabbit pAb (A2547)}

Western blot - NF-kB p65/RelA Rabbit pAb (A2547)

Western blot analysis of various lysates using NF-kB p65/RelA Rabbit pAb (A2547) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - NF-kB p65/RelA Rabbit pAb (A2547)}

Western blot - NF-kB p65/RelA Rabbit pAb (A2547)

Western blot analysis of various lysates, using NF-kB p65/RelA Rabbit pAb (A2547) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Western blot - NF-kB p65/RelA Rabbit pAb (A2547)}

Western blot - NF-kB p65/RelA Rabbit pAb (A2547)

Western blot analysis of lysates from wild type(WT) and NF-kB p65/RelA knockout (KO) HeLa(KO) cells, using NF-kB p65/RelA Rabbit pAb (A2547) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)}

Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry analysis of paraffin-embedded Human colon using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)}

Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)}

Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry analysis of paraffin-embedded Rat testis using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)}

Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry analysis of paraffin-embedded Human colon using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)}

Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry analysis of paraffin-embedded Mouse colon using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)}

Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A2547)

Immunohistochemistry analysis of paraffin-embedded Rat heart using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)}

Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of HepG2 cells using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)}

Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)}

Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of HepG2 cells using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)}

Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A2547)

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (14)

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Secondary Antibodies (25)