제품 > 항체 > 다중클론항체(pAb)

NOX1 Rabbit pAb (A8527)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using NOX1 Rabbit pAb (A8527) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name	NOX1 Rabbit pAb
Catalog No.	A8527
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 415-564 of human NOX1 (NP_008983.2).
Sequence	KSIWYKFQCADHNLKTKKIYFYWICRETGAFSWFNNLLTSLEQEMEELGKVGFLNYRLFLTGWDSNIVGHAALNFDKATDIVTGLKQKTSFGRPMWDNEFSTIATSHPKSVVGVFLCGPRTLAKSLRKCCHRYSSLDPRKVQFYFNKENF
Gene ID
Swiss Prot
Synonyms	MOX1; NOH1; NOH-1; GP91-2; NOH-1L; NOX1
Calculated MW	65kDa
Observed MW	65kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HT-29, SW480, Mouse liver, Rat brain
Cellular location	Cell projection, Multi-pass membrane protein, invadopodium membrane.
Customer validation	WB(Homo sapiens, Rattus norvegicus, Mus musculus) IHC(Mus musculus)

Research Area

NOX1 Rabbit pAb images

Western blot - NOX1 Rabbit pAb (A8527)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for NOX1 Rabbit pAb (A8527)

Product:

NOX1 Rabbit pAb

Journal:

Environmental pollution (Barking, Essex : 1987)

Application:

IF:

7.6

Species:

Homo sapiens

PMID:

31401526

Title:

PM2.5 induces ferroptosis in human endothelial cells through iron overload and redox imbalance.

References for NOX1 Rabbit pAb (A8527)

Product:

NOX1 Rabbit pAb

Journal:

Biochemical Pharmacology

Application:

IF:

5.3

Species:

Homo sapiens,Rattus norvegicus

PMID:

32251672

Title:

Dipeptidyl peptidase-4 inhibition improves endothelial senescence by activating AMPK/SIRT1/Nrf2 signaling pathway

References for NOX1 Rabbit pAb (A8527)

Product:

NOX1 Rabbit pAb

Journal:

Signal Transduct Target Ther

Application:

IHC

IF:

40.8

Species:

Mus musculus

PMID:

32792487

Title:

Membranous NOX5-derived ROS oxidizes and activates local Src to promote malignancy of tumor cells

References for NOX1 Rabbit pAb (A8527)

Product:

NOX1 Rabbit pAb

Journal:

J Hazard Mater

Application:

IF:

12.2

Species:

Homo sapiens

PMID:

34492938

Title:

Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells

References for NOX1 Rabbit pAb (A8527)

Product:

NOX1 Rabbit pAb

Journal:

Chemico-biological interactions

Application:

IF:

4.7

Species:

Mus musculus

PMID:

35661779

Title:

Ferroptosis is involved in the benzene-induced hematotoxicity in mice via iron metabolism, oxidative stress and NRF2 signaling pathway

References for NOX1 Rabbit pAb (A8527)

Product:

NOX1 Rabbit pAb

Journal:

Signal transduction and targeted therapy

Application:

IF:

40.8

Species:

Homo sapiens

PMID:

37160897

Title:

SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication - PMC

References for NOX1 Rabbit pAb (A8527)

Product:

NOX1 Rabbit pAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Mus musculus

PMID:

38104087

Title:

CB2 regulates oxidative stress and osteoclastogenesis through NOX1-dependent signaling pathway in titanium particle-induced osteolysis - PMC

References for NOX1 Rabbit pAb (A8527)

Product:

NOX1 Rabbit pAb

Journal:

Free radical biology & medicine

Application:

IF:

7.1

Species:

Mus musculus

PMID:

35398494

Title:

Old targets, new strategy: Apigenin-7-O--d-(-6-p-coumaroyl)-glucopyranoside prevents endothelial ferroptosis and alleviates intestinal ischemia-reperfusion injury through HO-1 and MAO-B inhibition

References for NOX1 Rabbit pAb (A8527)

Product:

NOX1 Rabbit pAb

Journal:

Cell communication and signaling : CCS

Application:

IF:

7.5

Species:

Homo sapiens

PMID:

39164737

Title:

ZMAT2 condensates regulate the alternative splicing of TRIM28 to reduce cellular ROS accumulation, thereby promoting the proliferation of HCC cells - PMC