제품 > 항체 > 다중클론항체(pAb)

NQO1 Rabbit pAb (A1518)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using NQO1 Rabbit pAb (A1518) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from Rat liver, using NQO1 Rabbit pAb (A1518) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - NQO1 Rabbit pAb (A1518)

Immunohistochemistry analysis of NQO1 in paraffin-embedded human colon carcinoma tissue using NQO1 Rabbit pAb (A1518) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of NQO1 in paraffin-embedded human liver tissue using NQO1 Rabbit pAb (A1518) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - NQO1 Rabbit pAb (A1518)

Immunofluorescence analysis of L929 cells using NQO1 Rabbit pAb (A1518) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	NQO1 Rabbit pAb
Catalog No.	A1518
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human NQO1 (NP_000894.1).
Sequence	MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK
Gene ID
Swiss Prot
Synonyms	DTD; QR1; DHQU; DIA4; NMOR1; NMORI; NQO1
Calculated MW	31kDa
Observed MW	31kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HepG2, MCF7, NIH/3T3，Rat liver
Cellular location	Cytoplasm
Customer validation	WB(Mus musculus, Homo sapiens, Gallus gallus)

Documents

Certificate of Compliance

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Research Area

NQO1 Rabbit pAb images

Western blot - NQO1 Rabbit pAb (A1518)

Immunohistochemistry - NQO1 Rabbit pAb (A1518)

Immunofluorescence - NQO1 Rabbit pAb (A1518)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for NQO1 Rabbit pAb (A1518)

Product:

NQO1 Rabbit pAb

Journal:

Antioxidants (Basel)

Application:

IF:

7.7

Species:

Mus musculus

PMID:

34439516

Title:

Luteolin Alleviates AflatoxinB 1-Induced Apoptosis and Oxidative Stress in the Liver of Mice through Activation of Nrf2 Signaling Pathway

References for NQO1 Rabbit pAb (A1518)

Product:

NQO1 Rabbit pAb

Journal:

Neuropharmacology

Application:

IF:

4.6

Species:

Mus musculus

PMID:

31874170

Title:

A novel biscoumarin compound ameliorates cerebral ischemia reperfusion-induced mitochondrial oxidative injury via Nrf2/Keap1/ARE signaling

References for NQO1 Rabbit pAb (A1518)

Product:

NQO1 Rabbit pAb

Journal:

Technol Cancer Res Treat

Application:

IF:

2.7

Species:

Homo sapiens

PMID:

33327894

Title:

Long Non-Coding RNA PVT1 Regulates the Resistance of the Breast Cancer Cell Line MDA-MB-231 to Doxorubicin via Nrf2

References for NQO1 Rabbit pAb (A1518)

Product:

NQO1 Rabbit pAb

Journal:

Journal of Experimental Medicine

Application:

IF:

12.6

Species:

Homo sapiens

PMID:

27091842

Title:

Gankyrin has an antioxidative role through the feedback regulation of Nrf2 in hepatocellular carcinoma.

References for NQO1 Rabbit pAb (A1518)

Product:

NQO1 Rabbit pAb

Journal:

Toxins

Application:

IF:

4.2

Species:

Gallus gallus

PMID:

30621062

Title:

Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB?-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers.

References for NQO1 Rabbit pAb (A1518)

Product:

NQO1 Rabbit pAb

Journal:

Int Immunopharmacol

Application:

IF:

4.8

Species:

Mus musculus

PMID:

33045565

Title:

Resveratrol alleviates oxidative stress caused by Streptococcus uberis infection via activating the Nrf2 signaling pathway

References for NQO1 Rabbit pAb (A1518)

Product:

NQO1 Rabbit pAb

Journal:

Journal of food biochemistry

Application:

IF:

Species:

Homo sapiens

PMID:

35894102

Title:

Protective effects of di‐caffeoylquinic acids from Artemisia selengensis Turcz leaves against monosodium urate‐induced inflammation via the modulation of NLRP3 …

References for NQO1 Rabbit pAb (A1518)

Product:

NQO1 Rabbit pAb

Journal:

ACS central science

Application:

IF:

12.7

Species:

Mus musculus

PMID:

36968547

Title:

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury - PMC

References for NQO1 Rabbit pAb (A1518)

Product:

NQO1 Rabbit pAb

Journal:

Scientific reports

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

37055457

Title:

CZK, a novel alkaloid derivative from Clausena lansium, alleviates ischemic stroke injury through Nrf2-mediated antioxidant effects - PMC

References for NQO1 Rabbit pAb (A1518)

Product:

NQO1 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

36948265

Title:

Rhus chinensis Mill. fruits alleviate liver injury induced by isoniazid and rifampicin through regulating oxidative stress, apoptosis, and bile acid transport

References for NQO1 Rabbit pAb (A1518)

Product:

NQO1 Rabbit pAb

Journal:

Chemico-biological interactions

Application:

IF:

4.7

Species:

Mus musculus

PMID:

37517432

Title:

Tangeretin and 4-demethyltangeretin prevent damage to mouse hepatocytes from oxidative stress by activating the Nrf2-related antioxidant pathway via an

References for NQO1 Rabbit pAb (A1518)

Product:

NQO1 Rabbit pAb

Journal:

Foods (Basel, Switzerland)

Application:

IF:

5.2

Species:

Mus musculus

PMID:

37628108

Title:

The Protective Effect and Mechanism of a Phytochemical Extract from the Wild Vegetable Shutou (Crateva unilocularis Buch.) against Acetaminophen-Induced Liver Injury in Mice - PMC