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NR2F2 Rabbit pAb (A10251)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NR2F2 Rabbit pAb (A10251)

Western blot analysis of various lysates, using NR2F2 Rabbit pAb (A10251) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - NR2F2 Rabbit pAb (A10251)

Western blot analysis of various lysates, using NR2F2 Rabbit pAb (A10251) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - NR2F2 Rabbit pAb (A10251)

Immunohistochemistry analysis of paraffin-embedded human kidney using NR2F2 Rabbit pAb (A10251) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NR2F2 Rabbit pAb (A10251)

Immunohistochemistry analysis of paraffin-embedded mouse liver using NR2F2 Rabbit pAb (A10251) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NR2F2 Rabbit pAb (A10251)

Immunohistochemistry analysis of paraffin-embedded rat liver using NR2F2 Rabbit pAb (A10251) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - NR2F2 Rabbit pAb (A10251)

Immunofluorescence analysis of A431 cells using NR2F2 Rabbit pAb (A10251) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNR2F2 Rabbit pAb
Catalog No.A10251
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human NR2F2 (NP_066285.1).
SequenceMAMVVSTWRDPQDEVPGSQGSQASQAPPVPGPPPGAPHTPQTPGQGGPASTPAQTAAGGQGGPGGPGSDKQQQQQHIECVVCGDKSSGKHYGQFTCEGCK
Gene ID
Swiss Prot
SynonymsARP1; ARP-1; CHTD4; NF-E3; SRXX5; SVP40; COUPTF2; COUPTFB; TFCOUP2; COUPTFII; NR2F2
Calculated MW46kDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, Molt-4(Negative control), Mouse liver, PC-12, C6
Cellular locationNucleus
Customer validation

IHC(Rattus norvegicus, Homo sapiens)

WB(Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - NR2F2 Rabbit pAb (A10251)}

Western blot - NR2F2 Rabbit pAb (A10251)

Western blot analysis of various lysates, using NR2F2 Rabbit pAb (A10251) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - NR2F2 Rabbit pAb (A10251)}

Western blot - NR2F2 Rabbit pAb (A10251)

Western blot analysis of various lysates, using NR2F2 Rabbit pAb (A10251) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - NR2F2 Rabbit pAb (A10251)}

Immunohistochemistry - NR2F2 Rabbit pAb (A10251)

Immunohistochemistry analysis of paraffin-embedded human kidney using NR2F2 Rabbit pAb (A10251) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NR2F2 Rabbit pAb (A10251)}

Immunohistochemistry - NR2F2 Rabbit pAb (A10251)

Immunohistochemistry analysis of paraffin-embedded mouse liver using NR2F2 Rabbit pAb (A10251) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NR2F2 Rabbit pAb (A10251)}

Immunohistochemistry - NR2F2 Rabbit pAb (A10251)

Immunohistochemistry analysis of paraffin-embedded rat liver using NR2F2 Rabbit pAb (A10251) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - NR2F2 Rabbit pAb (A10251)}

Immunofluorescence - NR2F2 Rabbit pAb (A10251)

Immunofluorescence analysis of A431 cells using NR2F2 Rabbit pAb (A10251) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A10251? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)