제품 > 항체 > 다중클론항체(pAb)

NRF1 Rabbit pAb (A5547)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using NRF1 Rabbit pAb (A5547) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Chromatin Immunoprecipitation - NRF1 Rabbit pAb (A5547)

Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using NRF1 antibody (A5547) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product name	NRF1 Rabbit pAb
Catalog No.	A5547
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 240-449 of human NRF1 (NP_001035199.1).
Sequence	WANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDAN
Gene ID
Swiss Prot
Synonyms	ALPHA-PAL; NRF1
Calculated MW	54kDa
Observed MW	70kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP 5μg antibody for 10μg-15μg of Chromatin
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HepG2, PC-3, Mouse brain, Mouse heart, Rat brain, Rat heart
Cellular location	Nucleus.
Customer validation	WB(Rattus norvegicus, Mus musculus, Homo sapiens, Danio rerio) IF(Danio rerio)

Documents

Certificate of Compliance

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Lot number

Research Area

NRF1 Rabbit pAb images

Western blot - NRF1 Rabbit pAb (A5547)

Chromatin Immunoprecipitation - NRF1 Rabbit pAb (A5547)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for NRF1 Rabbit pAb (A5547)

Product:

NRF1 Rabbit pAb

Journal:

Molecular Medicine Reports

Application:

IF:

2.952

Species:

Rattus norvegicus

PMID:

31702040

Title:

Astragaloside IV alleviates myocardial damage induced by type 2 diabetes via improving energy metabolism

References for NRF1 Rabbit pAb (A5547)

Product:

NRF1 Rabbit pAb

Journal:

The Science of the Total Environment

Application:

IF:

7.963

Species:

Mus musculus

PMID:

31733399

Title:

Mitochondrial damage are involved in Aflatoxin B1-induced testicular damage and spermatogenesis disorder in mice

References for NRF1 Rabbit pAb (A5547)

Product:

NRF1 Rabbit pAb

Journal:

Molecular Medicine Reports

Application:

IF:

2.952

Species:

Homo sapiens

PMID:

32377739

Title:

p53/PGC?1α?mediated Mitochondrial Dysfunction Promotes PC3 Prostate Cancer Cell Apoptosis.

References for NRF1 Rabbit pAb (A5547)

Product:

NRF1 Rabbit pAb

Journal:

Aging (Albany NY)

Application:

IF:

5.682

Species:

Mus musculus

PMID:

33231568

Title:

Pentoxifylline enhances antioxidative capability and promotes mitochondrial biogenesis for improving age-related behavioral deficits

References for NRF1 Rabbit pAb (A5547)

Product:

NRF1 Rabbit pAb

Journal:

Neuropharmacology

Application:

IF:

5.25

Species:

Rattus norvegicus

PMID:

34089729

Title:

Parecoxib alleviates the motor behavioral decline of aged rats by ameliorating mitochondrial dysfunction in the substantia nigra via COX-2/PGE2 pathway inhibition

References for NRF1 Rabbit pAb (A5547)

Product:

NRF1 Rabbit pAb

Journal:

iScience

Application:

IF:

6.1

Species:

Mus musculus

PMID:

37456842

Title:

Hepatic depletion of nucleolar protein mDEF causes excessive mitochondrial copper accumulation associated with p53 and NRF1 activation - PMC

References for NRF1 Rabbit pAb (A5547)

Product:

NRF1 Rabbit pAb

Journal:

Biology direct

Application:

IF:

7.17

Species:

Homo sapiens

PMID:

37875967

Title:

Nuclear respiratory factor 1 drives hepatocellular carcinoma progression by activating LPCAT1-ERK1/2-CREB axis - PMC

References for NRF1 Rabbit pAb (A5547)

Product:

NRF1 Rabbit pAb

Journal:

Frontiers in cell and developmental biology

Application:

WB, IF

IF:

6.08

Species:

Danio rerio

PMID:

37457295

Title:

Role of insulin-like growth factor 1 (IGF1) in the regulation of mitochondrial bioenergetics in zebrafish oocytes: lessons from in vivo and in vitro investigations - PMC

References for NRF1 Rabbit pAb (A5547)

Product:

NRF1 Rabbit pAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

7.31

Species:

Mus musculus

PMID:

35222795

Title:

Nrf2 Deficiency Attenuates Testosterone Efficiency in Ameliorating Mitochondrial Function of the Substantia Nigra in Aged Male Mice

References for NRF1 Rabbit pAb (A5547)

Product:

NRF1 Rabbit pAb

Journal:

Food science & nutrition

Application:

IF:

3.55

Species:

Mus musculus

PMID:

38268887

Title:

Lycopene protects against ionizing radiationinduced testicular damage by inhibition of apoptosis and mitochondrial dysfunction

References for NRF1 Rabbit pAb (A5547)

Product:

NRF1 Rabbit pAb

Journal:

Oxid Med Cell Longev

Application:

IF:

6.543

Species:

Mus musculus

PMID:

34257818

Title:

Pentoxifylline Enhances Antioxidative Capability and Promotes Mitochondrial Biogenesis in D-Galactose-Induced Aging Mice by Increasing Nrf2 and PGC-1 through the cAMP-CREB Pathway

References for NRF1 Rabbit pAb (A5547)

Product:

NRF1 Rabbit pAb

Journal:

Mediators of inflammation

Application:

IF:

4.529

Species:

Mus musculus

PMID:

35431653