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NRF2 Rabbit pAb (A0674)

Review (1)Publications (158) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NRF2 Rabbit pAb (A0674)

Western blot analysis of lysates from wild type (WT) and NRF2 knockout (KO) HeLa cells using NRF2 Rabbit pAb (A0674) at 1:1000 dilution. wild type (WT) and NRF2 knockout (KO) HeLa cells were treated by MG132(10 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A0674)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NRF2 Rabbit pAb (A0674) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A0674)

Immunohistochemistry analysis of paraffin-embedded Human placenta using NRF2 Rabbit pAb (A0674) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A0674)

Immunofluorescence analysis of Human pancreas tissue using NRF2 Rabbit pAb (A0674) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

Review
ABclonal: review for NRF2 Rabbit pAb(A0674)

Overview

Product nameNRF2 Rabbit pAb
Catalog No.A0674
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 243-400 of human NRF2 (NP_006155.2).
SequenceSPHFLNAFEDSFSSILSTEDPNQLTVNSLNSDATVNTDFGDEFYSAFIAEPSISNSMPSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSS
Gene ID
Swiss Prot
SynonymsNRF2; HEBP1; Nrf-2; IMDDHH
Calculated MW68kDa
Observed MW97-110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa treated by MG132
Cellular locationCytoplasm, Nucleus, cytosol.
Customer validation

(Mouse embryonal fibroblast cell)

WB(Gallus gallus, Homo sapiens, Mus musculus, Rattus norvegicus, quails, Vaccinium dunalianum, Hyline Brown layers, Sus scrofa, Pelteobagrus fulvidraco, Mus musculusRattus norvegicus, Rattus norvegicus , Mus musculus, Other, Rattus norvegicus)

IF(Rattus norvegicus, Mus musculus, Homo sapiens)

IHC(Mus musculus, Rattus norvegicus)

IP(Homo sapiens, Mus musculus)

IHC(Mus musculus, Homo sapiens, Rattus norvegicus, Other)

IF(Homo sapiens, Mus musculus, Rattus norvegicus, Mus musculus)

WB(Mus musculus, Rattus norvegicus)

IHC(Mus musculus)

Documents

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Lot number

ABclonal:Western blot - NRF2 Rabbit pAb (A0674)}

Western blot - NRF2 Rabbit pAb (A0674)

Western blot analysis of lysates from wild type (WT) and NRF2 knockout (KO) HeLa cells using NRF2 Rabbit pAb (A0674) at 1:1000 dilution. wild type (WT) and NRF2 knockout (KO) HeLa cells were treated by MG132(10 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A0674)}

Immunohistochemistry - NRF2 Rabbit pAb (A0674)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NRF2 Rabbit pAb (A0674) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A0674)}

Immunohistochemistry - NRF2 Rabbit pAb (A0674)

Immunohistochemistry analysis of paraffin-embedded Human placenta using NRF2 Rabbit pAb (A0674) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A0674)}

Immunofluorescence - NRF2 Rabbit pAb (A0674)

Immunofluorescence analysis of Human pancreas tissue using NRF2 Rabbit pAb (A0674) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (7)

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WBIF/ICCELISA

Reactivity:Human

Phospho-NRF2-S40 Rabbit pAb

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WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

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NRF2 Rabbit PolymAb®
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WBIF/ICCELISA

Reactivity:Human, Mouse, Rat

NRF2 Rabbit PolymAb®

ELISA 키트 (1)

Human NRF2 ELISA Kit
RK11523

Human NRF2 ELISA Kit

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)