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ODC1 Rabbit pAb (A1948)

Publication (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ODC1 Rabbit pAb (A1948)

Western blot analysis of various lysates using ODC1 Rabbit pAb (A1948) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - ODC1 Rabbit pAb (A1948)

Immunofluorescence analysis of NIH/3T3 cells using ODC1 Rabbit pAb (A1948) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ODC1 Rabbit pAb (A1948)

Immunofluorescence analysis of PC-12 cells using ODC1 Rabbit pAb (A1948) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ODC1 Rabbit pAb (A1948)

Immunofluorescence analysis of U2OS cells using ODC1 Rabbit pAb (A1948) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameODC1 Rabbit pAb
Catalog No.A1948
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ODC1 (NP_002530.1).
SequenceMNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGDILKKHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGTGFDCASKTEIQLVQSLGVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHPKAKLVLRIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFHVGSGCTDPETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLKFEEITGVINPALDKYFPSDSGVRIIAEPGRYYV
Gene ID
Swiss Prot
SynonymsODC; BABS; NEDBA; NEDBIA; ODC1
Calculated MW51kDa
Observed MW51kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, HepG2, Raji, Mouse kidney, Rat kidney
Cellular locationcytoplasm, cytosol
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - ODC1 Rabbit pAb (A1948)}

Western blot - ODC1 Rabbit pAb (A1948)

Western blot analysis of various lysates using ODC1 Rabbit pAb (A1948) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - ODC1 Rabbit pAb (A1948)}

Immunofluorescence - ODC1 Rabbit pAb (A1948)

Immunofluorescence analysis of NIH/3T3 cells using ODC1 Rabbit pAb (A1948) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ODC1 Rabbit pAb (A1948)}

Immunofluorescence - ODC1 Rabbit pAb (A1948)

Immunofluorescence analysis of PC-12 cells using ODC1 Rabbit pAb (A1948) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ODC1 Rabbit pAb (A1948)}

Immunofluorescence - ODC1 Rabbit pAb (A1948)

Immunofluorescence analysis of U2OS cells using ODC1 Rabbit pAb (A1948) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)