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Occludin Rabbit pAb (A2601)

Reviews (2)Publications (102) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Occludin Rabbit pAb
ABclonal:Western blot - Occludin Rabbit pAb (A2601)

Western blot analysis of lysates from Mouse liver cells using Occludin Rabbit pAb (A2601) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Occludin Rabbit pAb (A2601)

Immunofluorescence analysis of MCF7 cells using Occludin Rabbit pAb (A2601) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Occludin Rabbit pAb (A2601)

Immunofluorescence analysis of Human breast cancer tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - Occludin Rabbit pAb (A2601)

Immunofluorescence analysis of MCF7 cells using Occludin Rabbit pAb (A2601) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Occludin Rabbit pAb (A2601)

Immunofluorescence analysis of Human breast cancer tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

Review
ABclonal: review for Occludin Rabbit pAb(A2601)
Review
ABclonal: review for Occludin Rabbit pAb(A2601)

Overview

Product nameOccludin Rabbit pAb
Catalog No.A2601
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 400-522 of human Occludin (NP_002529.1).
SequenceTDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT
Gene ID
Swiss Prot
SynonymsBLCPMG; PTORCH1; PPP1R115; Occludin
Calculated MW59kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:1000 - 1:4000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse liver
Cellular locationCell junction, Membrane, Multi-pass membrane protein, tight junction.
Customer validation

(Mouse embryonal fibroblast cell, Pig cells)

WB(Sus scrofa, Rattus norvegicus, Mus musculus, Homo sapiens, Canis lupus familiaris, Mus musculus, Horabagrus nigricollaris, Macaca fascicularis)

IF(Mus musculus, Rattus norvegicus, Gallus gallus, Cyprinus carpio, Sus scrofa, Homo sapiens, Mus musculus)

IHC(Mus musculus, Ctenopharyngodon idellus, Oreochromis niloticus)

IF(Homo sapiens, Mus musculus)

IHC(Mus musculus, Rattus norvegicus, Horabagrus nigricollaris)

ELISA(Rattus norvegicus)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Occludin Rabbit pAb (A2601)}

Western blot - Occludin Rabbit pAb (A2601)

Western blot analysis of lysates from Mouse liver cells using Occludin Rabbit pAb (A2601) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)}

Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)}

Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)}

Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)}

Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)}

Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)}

Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)}

Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)}

Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)}

Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - Occludin Rabbit pAb (A2601)}

Immunofluorescence - Occludin Rabbit pAb (A2601)

Immunofluorescence analysis of MCF7 cells using Occludin Rabbit pAb (A2601) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Occludin Rabbit pAb (A2601)}

Immunofluorescence - Occludin Rabbit pAb (A2601)

Immunofluorescence analysis of Human breast cancer tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - Occludin Rabbit pAb (A2601)}

Immunofluorescence - Occludin Rabbit pAb (A2601)

Immunofluorescence analysis of MCF7 cells using Occludin Rabbit pAb (A2601) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Occludin Rabbit pAb (A2601)}

Immunofluorescence - Occludin Rabbit pAb (A2601)

Immunofluorescence analysis of Human breast cancer tissue using Occludin Rabbit pAb (A2601) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

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