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PD-L1/CD274 Rabbit pAb (A1645)

Reviews (2)Publications (34) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PD-L1/CD274 Rabbit pAb (A1645)

Western blot analysis of lysates from Mouse heart, using PD-L1/CD274 Rabbit pAb (A1645) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - PD-L1/CD274 Rabbit pAb (A1645)

Immunofluorescence analysis of paraffin-embedded human placenta using PD-L1/CD274 Rabbit pAb (A1645) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for PD-L1/CD274 Rabbit pAb(A1645)
Review
ABclonal: review for PD-L1/CD274 Rabbit pAb(A1645)

Overview

Product namePD-L1/CD274 Rabbit pAb
Catalog No.A1645
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 190-290 of human PD-L1/CD274 (NP_054862.1).
SequenceLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET
Gene ID
Swiss Prot
SynonymsB7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1; PD-L1/CD274
Calculated MW33kDa
Observed MW40-50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse heart
Cellular locationCell membrane, Endomembrane system, Single-pass type I membrane protein.
Customer validation

(Human gastric cancer tissue,Human gastric mucosa, Human liver cancer)

IHC(Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Mus musculus, Felis catus)

IHC(Homo sapiens)

IF(Mus musculus, Homo sapiens)

FC(Homo sapiens)

Other(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - PD-L1/CD274 Rabbit pAb (A1645)}

Western blot - PD-L1/CD274 Rabbit pAb (A1645)

Western blot analysis of lysates from Mouse heart, using PD-L1/CD274 Rabbit pAb (A1645) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - PD-L1/CD274 Rabbit pAb (A1645)}

Immunofluorescence - PD-L1/CD274 Rabbit pAb (A1645)

Immunofluorescence analysis of paraffin-embedded human placenta using PD-L1/CD274 Rabbit pAb (A1645) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (15)

재조합 단백질 (6)

제품 (4)

ELISA 키트 (1)

Secondary Antibodies (26)