Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from Mouse thymus, using PERK Rabbit pAb (A18196) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using PERK Rabbit pAb (A18196) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using PERK Rabbit pAb (A18196) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using PERK Rabbit pAb (A18196) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
| Product name | PERK Rabbit pAb |
|---|---|
| Catalog No. | A18196 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 867-1116 of human PERK (NP_004827.4). |
|---|---|
| Sequence | LTKNTTEKLQPSSPKVYLYIQMQLCRKENLKDWMNGRCTIEERERSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYARHTGQVGTKLYMSPEQIHGNSYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCEYVMVQDMLSPSPMERPEAINIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKHSRQSNNSHSPLPSN |
| Gene ID | |
| Swiss Prot | |
| Synonyms | PEK; WRS; PERK |
| Calculated MW | 125kDa |
| Observed MW | 140kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | Mouse thymus |
| Cellular location | Endoplasmic reticulum membrane, Single-pass type I membrane protein. |
| Customer validation | WB(Rattus norvegicus, Homo sapiens, Sus scrofa, Chlorocebus aethiops, Bos taurus, Mus musculus, Gallus gallus, Mus musculus, Misgurnus anguillicaudatus, Anatinae, Canis lupus familiaris) IF(Homo sapiens, Gallus gallus) WB(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A18196? Please let us know so that we can cite the reference in this datasheet.
_WB_01.jpg?t=1721354889 "ABclonal:Western blot - PERK Rabbit pAb (A18196)")
