제품 > 항체 > 다중클론항체(pAb)

PGC1α Rabbit pAb (A11971)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using PGC1α Rabbit pAb (A11971) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - PGC1α Rabbit pAb (A11971)

Immunohistochemistry analysis of paraffin-embedded Human kidney using PGC1α Rabbit pAb (A11971) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat kidney using PGC1α Rabbit pAb (A11971) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - PGC1α Rabbit pAb (A11971)

Immunofluorescence analysis of A-431 cells using PGC1α Rabbit pAb (A11971) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using PGC1α Rabbit pAb (A11971) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using PGC1α Rabbit pAb (A11971) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using PGC1α Rabbit pAb (A11971) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	PGC1α Rabbit pAb
Catalog No.	A11971
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 610-710 of human PGC1α (NP_037393.1).
Sequence	HRTHRNSPLYVRSRSRSPYSRRPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVFGEIEECTVNL
Gene ID
Swiss Prot
Synonyms	LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha); PGC1α
Calculated MW	91kDa
Observed MW	91kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Mouse kidney, Mouse heart, Rat heart
Cellular location	Cytoplasm, Nucleus, PML body.
Customer validation	WB(Mus musculus, Rattus norvegicus, Ctenopharyngodon idellus, Homo sapiens, Mus musculus, Gallus gallus) IF(Mus musculus, Rattus norvegicus) WB(Rattus norvegicus) IHC(Mus musculus) IF(Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

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PGC1α Rabbit pAb images

Western blot - PGC1α Rabbit pAb (A11971)

Immunohistochemistry - PGC1α Rabbit pAb (A11971)

Immunofluorescence - PGC1α Rabbit pAb (A11971)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Cell Stress Chaperones

Application:

IF:

3.66

Species:

Mus musculus

PMID:

33743152

Title:

Heat stress promotes lipid accumulation by inhibiting the AMPK-PGC-1α signaling pathway in 3T3-L1 preadipocytes

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Neuropharmacology

Application:

IF:

5.25

Species:

Rattus norvegicus

PMID:

34089729

Title:

Parecoxib alleviates the motor behavioral decline of aged rats by ameliorating mitochondrial dysfunction in the substantia nigra via COX-2/PGE2 pathway inhibition

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Oxid Med Cell Longev

Application:

IF:

6.543

Species:

Mus musculus

PMID:

34721761

Title:

Nrf2 Activation Attenuates Chronic Constriction Injury-Induced Neuropathic Pain via Induction of PGC-1 α-Mediated Mitochondrial Biogenesis in the Spinal Cord

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Cell metabolism

Application:

IF:

31.37

Species:

Mus musculus

PMID:

36525963

Title:

Discovery of a potent allosteric activator of DGKQ that ameliorates obesity-induced insulin resistance via the sn-1, 2-DAG-PKCε signaling axis

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

The British journal of nutrition

Application:

IF:

4.12

Species:

Ctenopharyngodon idellus

PMID:

35508921

Title:

Role of creatine supplementation on the myofiber characteristics and muscle protein synthesis of grass carp ( Ctenopharyngodon idellus )

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Journal of cardiology

Application:

IF:

2.97

Species:

Homo sapiens

PMID:

35570096

Title:

Ascending aortic perivascular adipose tissue inflammation associates with aortic valve disease

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Molecular metabolism

Application:

IF:

8.56

Species:

Mus musculus

PMID:

37321371

Title:

Salt-inducible kinase inhibition promotes the adipocyte thermogenic program and adipose tissue browning - PMC

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

7.67

Species:

Mus musculus

PMID:

37760023

Title:

PGC-1 Agonist Rescues Doxorubicin-Induced Cardiomyopathy by Mitigating the Oxidative Stress and Necroptosis - PMC

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Diabetes

Application:

IF:

9.33

Species:

Rattus norvegicus

PMID:

37196349

Title:

Leucine supplementation ameliorates early life "programming" of obesity in rats

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

The Journal of biological chemistry

Application:

IF:

Species:

Mus musculus

PMID:

37315788

Title:

Hepatic protein kinase Cbeta (PKC) deficiency mitigates late-onset obesity

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Journal of hazardous materials

Application:

IF:

14.22

Species:

Homo sapiens

PMID:

37604032

Title:

Daucosterol confers protection against T-2 toxin induced blood-brain barrier toxicity through the PGC-1-mediated defensive response in vitro and in vivo

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Application:

IF:

Species:

Rattus norvegicus

PMID:

38069169

Title:

Lactoferrin Alleviates Ethanol-Induced Injury via Promoting Nrf2 Nuclear Translocation in BRL-3A Rat Liver Cells - PMC

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

The Science of the total environment

Application:

IF:

10.75

Species:

Homo sapiens, Mus musculus

PMID:

36731555

Title:

Real-ambient particulate matter exposure induced FGFR1 methylation contributes to cardiac dysfunction by altering cardiac lipid metabolism

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Biochemical pharmacology

Application:

IF, WB

IF:

6.1

Species:

Rattus norvegicus

PMID:

38354958

Title:

PGC1 activation ameliorates cancer-induced bone pain via inhibiting apoptosis of GABAergic interneurons

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

6.1

Species:

Mus musculus

PMID:

38453725

Title:

Folic Acid Rescues Dopaminergic Neurons in MPTP-Induced Mice by Inhibiting the NLRP3 Inflammasome and Ameliorating Mitochondrial Impairment

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Life Sci

Application:

IF:

6.78

Species:

Rattus norvegicus

PMID:

33774032

Title:

Astragaloside IV-targeting miRNA-1 attenuates lipopolysaccharide-induced cardiac dysfunction in rats through inhibition of apoptosis and autophagy

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Oxid Med Cell Longev

Application:

IF:

6.543

Species:

Mus musculus

PMID:

34257818

Title:

Pentoxifylline Enhances Antioxidative Capability and Promotes Mitochondrial Biogenesis in D-Galactose-Induced Aging Mice by Increasing Nrf2 and PGC-1 through the cAMP-CREB Pathway

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Front Pharmacol

Application:

WB, IHC, IF

IF:

5.988

Species:

Mus musculus

PMID:

34588976

Title:

N-Acetyl Cysteine Ameliorates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease and Intracellular Triglyceride Accumulation by Preserving Mitochondrial Function

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Brain, behavior, and immunity

Application:

WB, IF

IF:

19.22

Species:

Rattus norvegicus

PMID:

35151829

Title:

Sestrin2 overexpression attenuates osteoarthritis pain via induction of AMPK/PGC-1-mediated mitochondrial biogenesis and suppression of neuroinflammation

References for PGC1α Rabbit pAb (A11971)

Product:

PGC1α Rabbit pAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

5.57

Species:

Mus musculus

PMID:

35697184