제품 > 항체 > 단일클론항체(mAb)

PI3 Kinase p110 delta Rabbit mAb (A19742)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse thymus, using PI3 Kinase p110 delta Rabbit mAb (A19742) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - PI3 Kinase p110 delta Rabbit mAb (A19742)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using PI3 Kinase p110 delta Rabbit mAb (A19742) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat spleen using PI3 Kinase p110 delta Rabbit mAb (A19742) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product name	PI3 Kinase p110 delta Rabbit mAb
Catalog No.	A19742
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2268

Background

Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I PI3K found primarily in leukocytes. Like other class I PI3Ks (p110-alpha p110-beta, and p110-gamma), the encoded protein binds p85 adapter proteins and GTP-bound RAS. However, unlike the other class I PI3Ks, this protein phosphorylates itself, not p85 protein.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human PI3 Kinase p110 delta (O00329).
Sequence	EESFTFQVSTKDVPLALMACALRKKATVFRQPLVEQPEDYTLQVNGRHEYLYGSYPLCQFQYICSCLHSGLTPHLTMVHSSSILAMRDEQSNPAPQVQKPR
Gene ID
Swiss Prot
Synonyms	APDS; PI3K; IMD14; p110D; IMD14A; IMD14B; ROCHIS; P110DELTA; PI3 Kinase p110 delta
Calculated MW	119kDa
Observed MW	119kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Mouse thymus
Cellular location	cytoplasm, cytosol, plasma membrane
Customer validation	WB(Rattus norvegicus, Mus musculus, Gallus gallus, Homo sapiens, Oryctolagus cuniculus, Other) IHC(Homo sapiens) IF(Homo sapiens) IP(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

PI3 Kinase p110 delta Rabbit mAb images

Western blot - PI3 Kinase p110 delta Rabbit mAb (A19742)

Immunohistochemistry - PI3 Kinase p110 delta Rabbit mAb (A19742)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

5.57

Species:

Rattus norvegicus

PMID:

35952824

Title:

Effect of propiconazole on neutrophil extracellular traps formation: Assessing the role of autophagy

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Mediators of inflammation

Application:

IF:

4.52

Species:

Mus musculus

PMID:

36133744

Title:

PI3K/AKT/mTOR Signaling Pathway Is Downregulated by Runzaoling (RZL) in Sjögren's Syndrome - PMC

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Free radical biology & medicine

Application:

IF:

8.1

Species:

Gallus gallus

PMID:

35841984

Title:

The decrease of selenoprotein K induced by selenium deficiency in diet improves apoptosis and cell progression block in chicken liver via the PTEN/PI3K/AKT …

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Journal of cancer research and clinical oncology

Application:

IF:

4.32

Species:

Homo sapiens

PMID:

36471019

Title:

Orelabrutinib and venetoclax synergistically induce cell death in double-hit lymphoma by interfering with the crosstalk between the PI3K/AKT and p38/MAPK signaling

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

7.41

Species:

Rattus norvegicus

PMID:

37245336

Title:

Areca catechu L. ameliorates chronic unpredictable mild stress-induced depression behavior in rats by the promotion of the BDNF signaling pathway

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Mus musculus

PMID:

37205911

Title:

Fresh Gastrodia elata Blume alleviates simulated weightlessness-induced cognitive impairment by regulating inflammatory and apoptosis-related pathways - PMC

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Current medicinal chemistry

Application:

IF:

4.74

Species:

Mus musculus

PMID:

37936460

Title:

Anti-migratory Properties of Cryoprotective Isoliquiritigenin-zein Phosphatidylcholine Nanoparticles Prevent Triple-negative Breast Cancer through PI3K-mTOR and

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Biochemical and biophysical research communications

Application:

WB, IHC, IF

IF:

3.32

Species:

Homo sapiens

PMID:

36621113

Title:

Single-cell transcriptome analysis identifies Versican (+) myofibroblast as a hallmark for thoracic aortic aneurysm marked by activation of PI3K-AKT signaling pathway

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Tissue & cell

Application:

WB, IP

IF:

2.58

Species:

Homo sapiens

PMID:

35926257

Title:

SIAH2 regulates colorectal cancer tumorigenesis via PI3K/ATK signaling pathway

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Cellular Signalling

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

36473621

Title:

DHCR7 promotes tumorigenesis via activating PI3K/AKT/mTOR signalling pathway in bladder cancer

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Human cell

Application:

IF:

4.37

Species:

Homo sapiens

PMID:

38133876

Title:

Hypothermic machine perfusion reduces donation after circulatory death liver ischemiareperfusion injury through the SERPINA3-mediated PI3K/Akt pathway

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Human cell

Application:

IF:

4.37

Species:

Homo sapiens

PMID:

38133876

Title:

Hypothermic machine perfusion reduces donation after circulatory death liver ischemiareperfusion injury through the SERPINA3-mediated PI3K/Akt pathway

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Small (Weinheim an der Bergstrasse, Germany)

Application:

IF:

13.3

Species:

Rattus norvegicus

PMID:

38778750

Title:

Extracellular VesicleContained Thrombospondin 1 Retards AgeRelated Degenerative Tendinopathy by Rejuvenating Tendon Stem/Progenitor Cell Senescence

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Small (Weinheim an der Bergstrasse, Germany)

Application:

IF:

13.3

Species:

Rattus norvegicus

PMID:

38778750

Title:

Extracellular VesicleContained Thrombospondin 1 Retards AgeRelated Degenerative Tendinopathy by Rejuvenating Tendon Stem/Progenitor Cell Senescence

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

International journal of pharmaceutics: X

Application:

IF:

4.7

Species:

Oryctolagus cuniculus

PMID:

39055743

Title:

Development and optimization of the Glabridin-loaded dissolving microneedle for enhanced treatment of keloid - PMC

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

The Journal of steroid biochemistry and molecular biology

Application:

IF:

4.1

Species:

Homo sapiens,Rattus norvegicus

PMID:

38909867

Title:

Investigation on the mechanism of androsta-4, 6, 8, 14-tetraene-3, 11, 16-trione against acute lymphoblastic leukemia

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Bioorganic chemistry

Application:

IF:

5.1

Species:

Homo sapiens

PMID:

38735265

Title:

Biological impact and therapeutic potential of a novel camptothecin derivative (FLQY2) in pancreatic cancer through inactivation of the PDK1/AKT/mTOR pathway

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Journal of clinical periodontology

Application:

IF:

6.7

Species:

Homo sapiens

PMID:

38379293

Title:

Isolation, identification and osteogenic capability analysis of mesenchymal stem cells derived from different layers of human maxillary sinus membrane

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Foods (Basel, Switzerland)

Application:

IF:

5.2

Species:

Other

PMID:

39410151

Title:

Characterization of the Composition of Bioactive Fractions from Dendrobium officinale Flowers That Protect against H2O2-Induced Oxidative Damage through the PI3K/AKT/Nrf2 Pathway - PMC

References for PI3 Kinase p110 delta Rabbit mAb (A19742)

Product:

PI3 Kinase p110 delta Rabbit mAb

Journal:

Journal of the science of food and agriculture

Application:

IF:

4.1

Species:

Mus musculus

PMID:

38445528