Western blot - PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM)
Western blot analysis of various lysates, using PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot - PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM)
Western blot analysis of lysates from Rat lung, using PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
Immunohistochemistry - PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM)
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM)
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM)
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM)
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM)
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.