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PLTP Rabbit mAb (A9644)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PLTP Rabbit mAb (A9644)

Western blot analysis of various lysates using PLTP Rabbit mAb (A9644) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PLTP Rabbit mAb (A9644)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using PLTP Rabbit mAb (A9644) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PLTP Rabbit mAb (A9644)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using PLTP Rabbit mAb (A9644) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product namePLTP Rabbit mAb
Catalog No.A9644
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1676
The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human PLTP (P55058).
SequenceLDTVPVRSSVDELVGIDYSLMKDPVASTSNLDMDFRGAFFPLTERNWSLPNRAVEPQLQEEERMVYVAFSEFFFDSAMESYFRAGALQLLLVGDKVPHDLD
Gene ID
Swiss Prot
SynonymsBPIFE; HDLCQ9; PLTP
Calculated MW55kDa
Observed MW70-80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:100 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse brain, Rat brain
Cellular locationextracellular region, extracellular space, nucleus.

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ABclonal:Western blot - PLTP Rabbit mAb (A9644)}

Western blot - PLTP Rabbit mAb (A9644)

Western blot analysis of various lysates using PLTP Rabbit mAb (A9644) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PLTP Rabbit mAb (A9644)}

Immunohistochemistry - PLTP Rabbit mAb (A9644)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using PLTP Rabbit mAb (A9644) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PLTP Rabbit mAb (A9644)}

Immunohistochemistry - PLTP Rabbit mAb (A9644)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using PLTP Rabbit mAb (A9644) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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